Evaluation of a new generation of plastic culture bottles with an automated microbial detection system for nine common contaminating organisms found in PLT components

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2004-03, Vol.44 (3), p.359-363
• Main Authors: Brecher, M.E., Hay, S.N., Rothenberg, S.J.
• Format: Article
• Language: English
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Bacillus cereus - isolation & purification; Bacterial Infections - prevention & control; Biological and medical sciences; Blood Component Removal; Blood Platelets; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; Enterobacter cloacae - isolation & purification; Escherichia coli - isolation & purification; Evaluation Studies as Topic; Glass; Humans; Klebsiella pneumoniae - isolation & purification; Medical sciences; Microbiological Techniques - instrumentation; Microbiological Techniques - methods; Plastics; Platelet Transfusion; Propionibacterium acnes - isolation & purification; Serratia marcescens - isolation & purification; Staphylococcus aureus - isolation & purification; Staphylococcus epidermidis - isolation & purification; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Viridans Streptococci - isolation & purification
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1111/j.1537-2995.2003.00617.x

BACKGROUND: A microbial detection system (BacT/ALERT 3D, bioMérieux [formerly Organon Teknika]) has previously been validated with a variety of bacterial contaminants in PLTs. The recovery of nine organisms seeded into PLTs with new plastic culture bottles was studied in comparison to the current glass bottles. The use of plastic instead of glass would be expected to reduce the risk of injury. STUDY DESIGN AND METHODS: Isolates of Bacillus cereus, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, Streptococcus viridans, and Propionibacterium acnes were inoculated into Day 2 (>24 hr