Cellular Proteins Bind to Sequence Motifs in the R1 Element between the HCMV Immune Evasion Genes

The viral US3 and US6 gene products of human cytomegalovirus (CMV) are sequentially expressed at immediate-early and early times after infection, respectively. They downregulate the surface expression of HLA class I molecules. There are two repeat-containing regulatory regions between the US3 promot...

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Bibliographic Details
Published in:Experimental and molecular pathology 2002-06, Vol.72 (3), p.196-206
Main Authors: Bullock, Grant C., Thrower, Abby R., Stinski, Mark F.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites - genetics
Biological and medical sciences
Cells, Cultured
Cytomegalovirus - genetics
Cytomegalovirus - immunology
Cytomegalovirus - metabolism
DNA, Viral - genetics
DNA, Viral - metabolism
DNA-Binding Proteins - metabolism
Experimental viral diseases and models
Gene Expression Regulation, Viral
Genes, Immediate-Early
Genes, Viral
Glycoproteins
HeLa Cells
Humans
Immediate-Early Proteins - genetics
Infectious diseases
Medical sciences
Membrane Proteins
Nuclear Proteins - metabolism
Protein Binding
RNA-Binding Proteins - genetics
Viral diseases
Viral Proteins - genetics
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Summary:The viral US3 and US6 gene products of human cytomegalovirus (CMV) are sequentially expressed at immediate-early and early times after infection, respectively. They downregulate the surface expression of HLA class I molecules. There are two repeat-containing regulatory regions between the US3 promoter and the US6 transcription unit designated R1 and R2. R2 contains repetitions of the NF-κB responsive element and enhances the immediate-early expression of the US3 gene. R1 contains 19 repetitions of a 5′-TRTCG-3′ pentanucleotide arranged as everted repeats, inverted repeats, and variably spaced single pentanucleotides. In the context of the viral genome, R1 also enhances immediate-early US3 gene expression by an unknown mechanism (G. C. Bullock, et al., 2001, Virology 288, 164–174). We report a sequence motif within the R1 element that binds a human cell nuclear protein which is antigenically related to the Drosophila boundary element-associated factor (BEAF). The potential role of a 35-kDa cellular protein that binds to sequence motifs within the R1 element in regulating the expression of the CMV US3 immune evasion gene is discussed.
ISSN:0014-4800
1096-0945
DOI:10.1006/exmp.2002.2428