Capacitance measurements at the calyx of Held in the medial nucleus of the trapezoid body

• Published in: Journal of neuroscience methods 2004-04, Vol.134 (2), p.121-131
• Main Authors: Sun, Jian-Yuan, Wu, Xin-Sheng, Wu, Wei, Jin, Shan-Xue, Dondzillo, Anna, Wu, Ling-Gang
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Axons - metabolism; Brain Stem - cytology; Brain Stem - physiology; Cadmium - pharmacology; Calyx of Held; Capacitance measurements; Computer Simulation; Electric Capacitance; Endocytosis; Excitatory Postsynaptic Potentials - physiology; Exocytosis; In Vitro Techniques; Isoquinolines - metabolism; Models, Neurological; Neurons - cytology; Neurons - drug effects; Neurons - physiology; Patch-Clamp Techniques - methods; Presynaptic; Rat; Rats; Rats, Wistar; Synapses - classification; Synapses - drug effects; Synapses - physiology; Time Factors
• ISSN: 0165-0270; 1872-678X
• DOI: 10.1016/j.jneumeth.2003.11.018

We have recently applied Lindau–Neher’s capacitance measurement technique to study vesicle trafficking at the calyx-type synapse in the rat medial nucleus of the trapezoid body (MNTB) in slice conditions. This application made the MNTB synapse an excellent model for the study of exocytosis and endocytosis at conventional active zones. However, the application was only made at calyces that are presumably equivalent to a single-compartment circuit because their passive current transients decayed mono-exponentially. Here, we determined whether the application could be extended to majority of calyces whose passive current transients decayed bi-exponentially. By comparison of calyces with mono- or bi-exponential decay in their passive current transients, we found similar properties in respect to: (1) the capacitance jump induced by trains of action-potential equivalent stimuli, which reflects exocytosis; (2) the size of a releasable vesicle pool; (3) the time course of the decay after the capacitance jump, which reflects endocytosis; and (4) the transient capacitance artifact observed in the presence of Cd 2+ that blocks exocytosis. These similar properties were also obtained from modeling calyces as a single- or two-compartment circuit. Thus, capacitance measurements may be extended to the majority of calyces, which may facilitate the study of rapid vesicle trafficking at conventional active zones.