High level of alpha1-acid glycoprotein in human seminal plasma is associated with high branching and expression of Lewis(a) groups on its glycans: supporting evidence for a prostatic origin

Changes in concentration of seminal plasma alpha(1)-acid glycoprotein (AGP) have been studied in detail before. However, the source of high levels of AGP as well as the glycosylation of seminal plasma AGP has not been elucidated yet. The glycosylation of AGP was studied by crossed affinity immunoele...

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Bibliographic Details
Published in:The Prostate 2002-06, Vol.52 (1), p.34-42
Main Authors: Poland, Dennis C W, Kratz, Ewa, Vermeiden, Jan P W, De Groot, Steffen M, Bruyneel, Ben, De Vries, Theodora, Van Dijk, Willem
Format: Article
Language:English
Subjects:
Amidohydrolases - metabolism
Carbohydrate Conformation
Fucosyltransferases - metabolism
Glycosylation
Humans
Immunoelectrophoresis, Two-Dimensional
Lectins
Lewis Blood-Group System - analysis
Lewis Blood-Group System - chemistry
Male
Molecular Weight
Monosaccharides - analysis
Orosomucoid - analysis
Orosomucoid - chemistry
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Plant Lectins
Polysaccharides - analysis
Polysaccharides - metabolism
Prostate - metabolism
Semen - chemistry
Semen - enzymology
Substrate Specificity
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Summary:Changes in concentration of seminal plasma alpha(1)-acid glycoprotein (AGP) have been studied in detail before. However, the source of high levels of AGP as well as the glycosylation of seminal plasma AGP has not been elucidated yet. The glycosylation of AGP was studied by crossed affinity immunoelectrophoresis using fucose-specific lectins and immunostaining. Glycan structure and monosaccharide analyses were performed by high pH anion exchange chromatography with pulsed amperometric detection. Fucosyltransferases were analyzed for activity and their substrate specificity was determined. Two types of fucosylation were detected; Lewis(x) and Lewis(a). Lewis(a) groups were only present on AGP of individuals with a high concentration and were completely absent when the AGP concentration in seminal plasma was low. Lewis(a) expression coincides with a higher degree of branching of the glycans and a relative increased alpha4-fucosyltransferase activity. The molecular weight of all seminal plasma AGP was slightly higher than of blood plasma AGP (approx. 47 vs. 41-43 kDa). The results indicate that AGP in seminal fluid most likely originates from the prostate and that it is either alpha3- or alpha4-fucosylated.
ISSN:0270-4137