Single LDL apheresis improves serum remnant-like particle-cholesterol, C-reactive protein, and malondialdehyde-modified-low-density lipoprotein concentrations in Japanese hypercholesterolemic subjects

• Published in: Clinica chimica acta 2002-07, Vol.321 (1), p.107-112
• Main Authors: Kobayashi, Junji, Katsube, Susumu, Shimoda, Mayumi, Furuhashi, Kenji, Kitano, Shouichi, Masuda, Mizue, Maruyama, Tokiko, Shinomiya, Masaki
• Format: Article
• Language: English
• Subjects: Apolipoproteins - blood; Biological and medical sciences; Blood Component Removal - methods; Body Mass Index; C-reactive protein (CRP); C-Reactive Protein - analysis; Cholesterol - blood; Disorders of blood lipids. Hyperlipoproteinemia; Familial hypercholesterolemia; Female; Fibrinogen - analysis; Humans; Hyperlipoproteinemia Type II - blood; Hyperlipoproteinemia Type II - metabolism; Hyperlipoproteinemia Type II - therapy; Japan; LDL-apheresis; Lipoproteins - blood; Lipoproteins, LDL - blood; Male; Malondialdehyde - metabolism; Malondialdehyde-modified-LDL (MDA-LDL); Medical sciences; Metabolic diseases; Middle Aged; Remnant-like particle-cholesterol (RLP-C); Triglycerides - blood
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/S0009-8981(02)00103-1

Background: Single low-density lipoprotein (LDL)-apheresis may affect serum remnant-like particle-cholesterol (RLP-C), C-reactive protein (CRP) and malondialdehyde-modified (MDA)-LDL concentrations. Subjects and methods: Six subjects with hypercholesterolemia (five men, one woman) were involved in this study. Mean age and body mass index of the study subjects were 58±3.1 years and 23.6±2.07 kg/m 2, respectively. Five of the subjects were diagnosed as heterozygous familial hypercholesterolemia (FH) because of having both marked hypercholesterolemia and Achilles tendon xanthomas. LDL apheresis was introduced and continued using a dextran sulfate cellulose adsorption column technique every 2 weeks. Serum RLP-C was measured using an immunoaffinity mixed gel containing anti-apolipoprotein A-I and anti-apolipoprotein B monoclonal antibody. Serum CRP was measured by latex-enhanced assay. Serum MDA-LDL was measured using monoclonal antibody against MDA-LDL (ML25). Results: Combined treatment in the steady state pre-treatment yielded a total, LDL- and HDL-cholesterol, and TG concentrations of 5.39±0.81, 3.82±1.03, 1.24±0.29 and 0.92±0.43 mmol/l, respectively, and a post-treatment total, LDL- and HDL-cholesterol and TG concentrations of 2.79±0.37 (−48%, p