Expression of glycoprotein 90K in human malignant pleural mesothelioma: correlation with patient survival

The expression of the tumour‐associated glycoprotein 90K in patients with malignant pleural mesothelioma (MM) has not been described. This study used enzyme‐linked immunoassay (ELISA) to measure 90K in pleural effusions (PEs) and sera from patients with MM (n=28), lung cancer (LC) (n=14) and benign...

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Bibliographic Details
Published in:The Journal of pathology 2002-06, Vol.197 (2), p.218-223
Main Authors: Strizzi, L., Muraro, R., Vianale, G., Natoli, C., Talone, L., Catalano, A., Mutti, L., Tassi, G., Procopio, A.
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens, Neoplasm
Biological and medical sciences
Biomarkers, Tumor - analysis
Blotting, Western
Carrier Proteins
diagnosis
Enzyme-Linked Immunosorbent Assay
Female
glycoprotein 90K
Glycoproteins - analysis
Humans
Male
Medical sciences
mesothelioma
Mesothelioma - chemistry
Mesothelioma - diagnosis
Middle Aged
Neoplasm Proteins - analysis
Pleural Effusion, Malignant - chemistry
Pleural Effusion, Malignant - diagnosis
Pleural Neoplasms - chemistry
Pleural Neoplasms - diagnosis
Pneumology
Prognosis
Survival Rate
Tumor Cells, Cultured
Tumors of the respiratory system and mediastinum
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Summary:The expression of the tumour‐associated glycoprotein 90K in patients with malignant pleural mesothelioma (MM) has not been described. This study used enzyme‐linked immunoassay (ELISA) to measure 90K in pleural effusions (PEs) and sera from patients with MM (n=28), lung cancer (LC) (n=14) and benign pleural disease (BPD) (n=15). Immunohistochemistry was used to investigate 90K expression in MM and LC tissue sections. The expression of 90K was further evaluated in vitro by ELISA and western blot analysis of conditioned media and cellular extracts of MM, LC and normal human mesothelial (NHM) cell cultures. Finally, the relationships between 90K expression in MM and patient age and survival were studied. The mean 90K level was significantly higher (p
ISSN:0022-3417
1096-9896
DOI:10.1002/path.1125