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Capillary electrophoretic method for determination of protease inhibitor indinavir sulfate used in human immunodeficiency virus therapy
A simple, fast and reliable capillary electrophoresis (CE) method for determination of indinavir sulfate, a potent protease inhibitor used in human immunodeficiency virus (HIV) therapy, in commercial and simulated capsule formulations is described. The analysis was performed in a 75 μm i.d. uncoated...
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| Published in: | Journal of pharmaceutical and biomedical analysis 2004-02, Vol.34 (2), p.441-450 |
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| cited_by | cdi_FETCH-LOGICAL-c422t-37b54e3728ff2692edd8465b34e8f1d4752eb18c886821f893001be48aae64aa3 |
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| cites | cdi_FETCH-LOGICAL-c422t-37b54e3728ff2692edd8465b34e8f1d4752eb18c886821f893001be48aae64aa3 |
| container_end_page | 450 |
| container_issue | 2 |
| container_start_page | 441 |
| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Aurora Prado, Marı́a S Kedor-Hackmann, Erika R.M Santoro, Maria Inês R.M Pinto, Terezinha J.A Tavares, Marina F.M |
| description | A simple, fast and reliable capillary electrophoresis (CE) method for determination of indinavir sulfate, a potent protease inhibitor used in human immunodeficiency virus (HIV) therapy, in commercial and simulated capsule formulations is described. The analysis was performed in a 75
μm i.d. uncoated fused-silica capillary with 27
cm length (effective length of 19.4
cm) using a 20
mmol
l
−1 phosphate buffer at pH 2.52. Samples were injected hydrodynamically by applying 0.5
psi pressure during 2
s. The applied voltage was 28
kV. Direct UV detection at 214
nm led to an adequate sensitivity without interference from sample excipients and known impurities. For quantitative purposes, diazepam was used as internal standard. Under optimized conditions, the migration times for indinavir sulfate and diazepam were 1.06 and 1.66
min, respectively. Analytical curve of peak area ratios
versus concentration in the range of 20.0–100.0
μg/ml gave a coefficient of correlation of 0.9992, establishing the method linearity. The limits of detection and quantitation were 4.61 and 14.0
μg/ml, respectively. The within-day precision expressed as relative standard deviation was |
| doi_str_mv | 10.1016/S0731-7085(03)00530-2 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71721094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708503005302</els_id><sourcerecordid>19259706</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-37b54e3728ff2692edd8465b34e8f1d4752eb18c886821f893001be48aae64aa3</originalsourceid><addsrcrecordid>eNqFkcuKFTEQhoMoznH0EZRsFF205tbd6ZXIwRsMuFDBXUgnFTrSnbRJeuA8ga9tzgV1N6uC8FWq6v8QekrJa0po9-Yr6TlteiLbl4S_IqTlpGH30I7KnjesEz_uo91f5Ao9yvknqRQdxEN0RVtCOW2HHfq916ufZ50OGGYwJcV1igmKN3iBMkWLXUzYQoG0-KCLjwFHh9cUC-gM2IfJj75UxgdbgVufcN5mpwvgLYOtz3jaFh2wX5YtRAvOGw_BHHBFt4zLBEmvh8fogdNzhieXeo2-f3j_bf-pufny8fP-3U1jBGOl4f3YCuA9k86xbmBgrRRdO3IB0lEr-pbBSKWRspOMOjlwQugIQmoNndCaX6MX53_rBb82yEUtPhuoCQSIW1Y97Rklg7gTpANrh550FWzPoEkx5wROrckvNVBFiTqqUidV6uhBEa5OqhSrfc8uA7ZxAfuv6-KmAs8vgM5Gzy7pYHz-j6vXUnrc9O2Zg5rbrYek8ilhsD5Vo8pGf8cqfwCiQbN9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19259706</pqid></control><display><type>article</type><title>Capillary electrophoretic method for determination of protease inhibitor indinavir sulfate used in human immunodeficiency virus therapy</title><source>Elsevier</source><creator>Aurora Prado, Marı́a S ; Kedor-Hackmann, Erika R.M ; Santoro, Maria Inês R.M ; Pinto, Terezinha J.A ; Tavares, Marina F.M</creator><creatorcontrib>Aurora Prado, Marı́a S ; Kedor-Hackmann, Erika R.M ; Santoro, Maria Inês R.M ; Pinto, Terezinha J.A ; Tavares, Marina F.M</creatorcontrib><description>A simple, fast and reliable capillary electrophoresis (CE) method for determination of indinavir sulfate, a potent protease inhibitor used in human immunodeficiency virus (HIV) therapy, in commercial and simulated capsule formulations is described. The analysis was performed in a 75
μm i.d. uncoated fused-silica capillary with 27
cm length (effective length of 19.4
cm) using a 20
mmol
l
−1 phosphate buffer at pH 2.52. Samples were injected hydrodynamically by applying 0.5
psi pressure during 2
s. The applied voltage was 28
kV. Direct UV detection at 214
nm led to an adequate sensitivity without interference from sample excipients and known impurities. For quantitative purposes, diazepam was used as internal standard. Under optimized conditions, the migration times for indinavir sulfate and diazepam were 1.06 and 1.66
min, respectively. Analytical curve of peak area ratios
versus concentration in the range of 20.0–100.0
μg/ml gave a coefficient of correlation of 0.9992, establishing the method linearity. The limits of detection and quantitation were 4.61 and 14.0
μg/ml, respectively. The within-day precision expressed as relative standard deviation was <1.5% for 10 consecutive sample injections. An average recovery of 100.81±0.56% at three concentration levels was obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of indinavir sulfate in capsule formulations, presenting additional advantages inherent to the CE technology, such as low consumption of reagents and column endurance.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(03)00530-2</identifier><identifier>PMID: 15013159</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Capillary electrophoresis ; Chemistry, Pharmaceutical ; Electrophoresis, Capillary - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HIV ; HIV Infections - drug therapy ; HIV Protease Inhibitors - analysis ; HIV Protease Inhibitors - chemistry ; HIV Protease Inhibitors - therapeutic use ; Human immunodeficiency virus ; Indinavir - analysis ; Indinavir - chemistry ; Indinavir - therapeutic use ; Indinavir sulfate ; Medical sciences ; Pharmacology. Drug treatments ; Protease inhibitor</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2004-02, Vol.34 (2), p.441-450</ispartof><rights>2003 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-37b54e3728ff2692edd8465b34e8f1d4752eb18c886821f893001be48aae64aa3</citedby><cites>FETCH-LOGICAL-c422t-37b54e3728ff2692edd8465b34e8f1d4752eb18c886821f893001be48aae64aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15475114$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15013159$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aurora Prado, Marı́a S</creatorcontrib><creatorcontrib>Kedor-Hackmann, Erika R.M</creatorcontrib><creatorcontrib>Santoro, Maria Inês R.M</creatorcontrib><creatorcontrib>Pinto, Terezinha J.A</creatorcontrib><creatorcontrib>Tavares, Marina F.M</creatorcontrib><title>Capillary electrophoretic method for determination of protease inhibitor indinavir sulfate used in human immunodeficiency virus therapy</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A simple, fast and reliable capillary electrophoresis (CE) method for determination of indinavir sulfate, a potent protease inhibitor used in human immunodeficiency virus (HIV) therapy, in commercial and simulated capsule formulations is described. The analysis was performed in a 75
μm i.d. uncoated fused-silica capillary with 27
cm length (effective length of 19.4
cm) using a 20
mmol
l
−1 phosphate buffer at pH 2.52. Samples were injected hydrodynamically by applying 0.5
psi pressure during 2
s. The applied voltage was 28
kV. Direct UV detection at 214
nm led to an adequate sensitivity without interference from sample excipients and known impurities. For quantitative purposes, diazepam was used as internal standard. Under optimized conditions, the migration times for indinavir sulfate and diazepam were 1.06 and 1.66
min, respectively. Analytical curve of peak area ratios
versus concentration in the range of 20.0–100.0
μg/ml gave a coefficient of correlation of 0.9992, establishing the method linearity. The limits of detection and quantitation were 4.61 and 14.0
μg/ml, respectively. The within-day precision expressed as relative standard deviation was <1.5% for 10 consecutive sample injections. An average recovery of 100.81±0.56% at three concentration levels was obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of indinavir sulfate in capsule formulations, presenting additional advantages inherent to the CE technology, such as low consumption of reagents and column endurance.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Capillary electrophoresis</subject><subject>Chemistry, Pharmaceutical</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HIV</subject><subject>HIV Infections - drug therapy</subject><subject>HIV Protease Inhibitors - analysis</subject><subject>HIV Protease Inhibitors - chemistry</subject><subject>HIV Protease Inhibitors - therapeutic use</subject><subject>Human immunodeficiency virus</subject><subject>Indinavir - analysis</subject><subject>Indinavir - chemistry</subject><subject>Indinavir - therapeutic use</subject><subject>Indinavir sulfate</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Protease inhibitor</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkcuKFTEQhoMoznH0EZRsFF205tbd6ZXIwRsMuFDBXUgnFTrSnbRJeuA8ga9tzgV1N6uC8FWq6v8QekrJa0po9-Yr6TlteiLbl4S_IqTlpGH30I7KnjesEz_uo91f5Ao9yvknqRQdxEN0RVtCOW2HHfq916ufZ50OGGYwJcV1igmKN3iBMkWLXUzYQoG0-KCLjwFHh9cUC-gM2IfJj75UxgdbgVufcN5mpwvgLYOtz3jaFh2wX5YtRAvOGw_BHHBFt4zLBEmvh8fogdNzhieXeo2-f3j_bf-pufny8fP-3U1jBGOl4f3YCuA9k86xbmBgrRRdO3IB0lEr-pbBSKWRspOMOjlwQugIQmoNndCaX6MX53_rBb82yEUtPhuoCQSIW1Y97Rklg7gTpANrh550FWzPoEkx5wROrckvNVBFiTqqUidV6uhBEa5OqhSrfc8uA7ZxAfuv6-KmAs8vgM5Gzy7pYHz-j6vXUnrc9O2Zg5rbrYek8ilhsD5Vo8pGf8cqfwCiQbN9</recordid><startdate>20040204</startdate><enddate>20040204</enddate><creator>Aurora Prado, Marı́a S</creator><creator>Kedor-Hackmann, Erika R.M</creator><creator>Santoro, Maria Inês R.M</creator><creator>Pinto, Terezinha J.A</creator><creator>Tavares, Marina F.M</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040204</creationdate><title>Capillary electrophoretic method for determination of protease inhibitor indinavir sulfate used in human immunodeficiency virus therapy</title><author>Aurora Prado, Marı́a S ; Kedor-Hackmann, Erika R.M ; Santoro, Maria Inês R.M ; Pinto, Terezinha J.A ; Tavares, Marina F.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-37b54e3728ff2692edd8465b34e8f1d4752eb18c886821f893001be48aae64aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Capillary electrophoresis</topic><topic>Chemistry, Pharmaceutical</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HIV</topic><topic>HIV Infections - drug therapy</topic><topic>HIV Protease Inhibitors - analysis</topic><topic>HIV Protease Inhibitors - chemistry</topic><topic>HIV Protease Inhibitors - therapeutic use</topic><topic>Human immunodeficiency virus</topic><topic>Indinavir - analysis</topic><topic>Indinavir - chemistry</topic><topic>Indinavir - therapeutic use</topic><topic>Indinavir sulfate</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Protease inhibitor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aurora Prado, Marı́a S</creatorcontrib><creatorcontrib>Kedor-Hackmann, Erika R.M</creatorcontrib><creatorcontrib>Santoro, Maria Inês R.M</creatorcontrib><creatorcontrib>Pinto, Terezinha J.A</creatorcontrib><creatorcontrib>Tavares, Marina F.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aurora Prado, Marı́a S</au><au>Kedor-Hackmann, Erika R.M</au><au>Santoro, Maria Inês R.M</au><au>Pinto, Terezinha J.A</au><au>Tavares, Marina F.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary electrophoretic method for determination of protease inhibitor indinavir sulfate used in human immunodeficiency virus therapy</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2004-02-04</date><risdate>2004</risdate><volume>34</volume><issue>2</issue><spage>441</spage><epage>450</epage><pages>441-450</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A simple, fast and reliable capillary electrophoresis (CE) method for determination of indinavir sulfate, a potent protease inhibitor used in human immunodeficiency virus (HIV) therapy, in commercial and simulated capsule formulations is described. The analysis was performed in a 75
μm i.d. uncoated fused-silica capillary with 27
cm length (effective length of 19.4
cm) using a 20
mmol
l
−1 phosphate buffer at pH 2.52. Samples were injected hydrodynamically by applying 0.5
psi pressure during 2
s. The applied voltage was 28
kV. Direct UV detection at 214
nm led to an adequate sensitivity without interference from sample excipients and known impurities. For quantitative purposes, diazepam was used as internal standard. Under optimized conditions, the migration times for indinavir sulfate and diazepam were 1.06 and 1.66
min, respectively. Analytical curve of peak area ratios
versus concentration in the range of 20.0–100.0
μg/ml gave a coefficient of correlation of 0.9992, establishing the method linearity. The limits of detection and quantitation were 4.61 and 14.0
μg/ml, respectively. The within-day precision expressed as relative standard deviation was <1.5% for 10 consecutive sample injections. An average recovery of 100.81±0.56% at three concentration levels was obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of indinavir sulfate in capsule formulations, presenting additional advantages inherent to the CE technology, such as low consumption of reagents and column endurance.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15013159</pmid><doi>10.1016/S0731-7085(03)00530-2</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2004-02, Vol.34 (2), p.441-450 |
| issn | 0731-7085 1873-264X |
| language | eng |
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| source | Elsevier |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Capillary electrophoresis Chemistry, Pharmaceutical Electrophoresis, Capillary - methods Fundamental and applied biological sciences. Psychology General pharmacology HIV HIV Infections - drug therapy HIV Protease Inhibitors - analysis HIV Protease Inhibitors - chemistry HIV Protease Inhibitors - therapeutic use Human immunodeficiency virus Indinavir - analysis Indinavir - chemistry Indinavir - therapeutic use Indinavir sulfate Medical sciences Pharmacology. Drug treatments Protease inhibitor |
| title | Capillary electrophoretic method for determination of protease inhibitor indinavir sulfate used in human immunodeficiency virus therapy |
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