Observations on experimental marginal periodontitis in rats

• Published in: Journal of periodontal research 2004-04, Vol.39 (2), p.101-106
• Main Authors: Kuhr, A., Popa-Wagner, A., Schmoll, H., Schwahn, C., Kocher, T.
• Format: Article
• Language: English
• Subjects: Age Factors; Alveolar Bone Loss - pathology; Alveolar Process - pathology; animal model; Animals; Biological and medical sciences; Dentistry; Disease Models, Animal; Disease Progression; experimental periodontal disease; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Ligation - instrumentation; ligature; Linear Models; Male; Medical sciences; Molar - pathology; Non tumoral diseases; Otorhinolaryngology. Stomatology; Periodontitis - physiopathology; Periodontium - physiopathology; Rats; Rats, Sprague-Dawley; Time Factors; Tooth Cervix - pathology; Tooth Root - pathology
• ISSN: 0022-3484; 1600-0765
• DOI: 10.1111/j.1600-0765.2004.00710.x

Objective:  The purpose of this study was to assess periodontal destruction following experimentally induced marginal periodontitis in rats by ligatures over a 60‐day observation period. The extent to which the physiological movement of teeth influenced the effect of the ligatures was also examined. In addition, two methods for measuring bone loss in the defleshed jaw were compared. Methods:  Thirty‐five male Sprague‐Dawley rats (SD) were divided into five groups. Marginal periodontitis was induced by ligatures on the second maxillary molars. Rats were killed after 15, 30, and 60 days. Rats in the control group were killed on day 1 and day 60. Bone loss was determined with two different methods on the buccal and palatinal surfaces of the defleshed jaw. In the first method, the distance of the cementoenamel junction (CEJ) from the alveolar bone crest (ABC) was measured at different sites; in the second method, the area of the exposed root surface of the molars was measured. Results:  Comparison of the control groups from day 1 and day 60 using both measuring methods showed significant differences in bone loss. In the area where the ligature was located, test rats exhibited significantly greater bone loss than control rats. Comparison of control rats from day 1 with test rats from day 15 showed that the increase in bone loss between the groups within the area of the ligature was significantly greater than outside it. The age‐dependent bone loss increases over the entire observation period of 60 days. The ligature‐induced bone loss increased most from day 1 to day 15; on days 30 and 60, slighter increases in bone loss were observed. Conclusions:  The application of this model can only be recommended for short (≤15 days) observation periods. The distance method should be preferred to the area method.