Angiogenesis investigations in tissue engineering. The cylinder model on the chorioallantois membrane

Tissue engineering (TE) applications include the isolation, culture, and seeding of cells into a suitable matrix or scaffold prior to in vivo transplantation. After transplantation, vascularization of the scaffold is a principal factor limiting cell viability for the first 6-8 days post transplantat...

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Bibliographic Details
Published in:Chirurg 2004-03, Vol.75 (3), p.284-290
Main Authors: Borges, J, Tegtmeier, F T, Torio-Padron, N, Mueller, M C, Stark, G B
Format: Article
Language:ger
Subjects:
Adipocytes - cytology
Adipocytes - transplantation
Allantois - blood supply
Animals
Capillaries - cytology
Cell Differentiation - physiology
Cell Division - physiology
Cell Survival - physiology
Chick Embryo
Chorion - blood supply
Endothelium, Vascular - cytology
Humans
Neovascularization, Physiologic - physiology
Stem Cell Transplantation
Tissue Engineering - methods
Transplantation, Heterologous
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Summary:Tissue engineering (TE) applications include the isolation, culture, and seeding of cells into a suitable matrix or scaffold prior to in vivo transplantation. After transplantation, vascularization of the scaffold is a principal factor limiting cell viability for the first 6-8 days post transplantation. A model has been developed for systematic analysis of this process. Fertilized white Leghorn eggs were incubated and opened at day 3 of incubation. Preadipocyte-seeded fibrin constructs were implanted in a specially designed plastic cylinder and placed through the opening onto the surface of the chorioallantoic membrane (CAM) at day 8 of incubation. Vascularization of the constructs by chorioallantoic blood vessels was assessed for up to 8 days post transplantation. The survival rate for embryos receiving constructs was about 90%. Histology confirmed transplant cell viability at day 4 post transplantation, and vascularization of the constructs by avian endothelial cells progressively increased thereafter. A new in vivo model to study the effect of angiogenesis in TE constructs including assessments of viability, proliferation, and differentiation of transplanted cells and biomaterial properties is presented. Advantages include easy access to the CAM vascular network, lack of immunocompetence, low cost, and avoidance of animal experiments.
ISSN:0009-4722
DOI:10.1007/s00104-003-0770-z