A simple, high throughput method for the quantification of sodium alginates on oesophageal mucosa

Sodium alginate is a potential bioadhesive, but the lack of a convenient and suitable method for its quantification on the mucosal surface complicates the evaluation of its mucosal retentive properties. This paper develops and evaluates a spectrophotometric method for the rapid quantification of a r...

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Bibliographic Details
Published in:European journal of pharmaceutics and biopharmaceutics 2004-03, Vol.57 (2), p.299-305
Main Authors: Richardson, J.C, Dettmar, P.W, Hampson, F.C, Melia, C.D
Format: Article
Language:English
Subjects:
Alginate
Alginates - analysis
Alginates - chemistry
Animals
Bioadhesion
Chromatography, High Pressure Liquid - methods
Dimethyl methylene blue
Esophagus - chemistry
Glucuronic Acid - analysis
Glucuronic Acid - chemistry
Hexuronic Acids - analysis
Hexuronic Acids - chemistry
Mucoadhesion
Mucosa
Mucous Membrane - chemistry
Oesophagus
Quantification
Swine
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Summary:Sodium alginate is a potential bioadhesive, but the lack of a convenient and suitable method for its quantification on the mucosal surface complicates the evaluation of its mucosal retentive properties. This paper develops and evaluates a spectrophotometric method for the rapid quantification of a range of sodium alginates differing in chemical composition, and investigates how quantification was influenced by the presence of oesophageal mucosa. The method, based on dye complexation with 1,9-dimethyl methylene blue (DMMB) was sensitive to alginate molecular weight and uronic acid composition, however, no significant correlations between assay performance and alginate molecular characteristics were demonstrated. The assay was also influenced by complexation time, calcium ions and mucin, but was unaffected by the presence of oesophageal tissue scrapings. The assay proved to be capable of quantifying sodium alginate with excellent linearity ( r=0.999), reproducibility (CV
ISSN:0939-6411
1873-3441
DOI:10.1016/S0939-6411(03)00150-4