Effect of hyperosmotic shock on phosphoenolpyruvate carboxykinase gene expression and gluconeogenic activity in the crab muscle

Chasmagnathus granulata phosphoenolpyruvate carboxykinase (PEPCK) cDNA from jaw muscle was cloned and sequenced, showing a specific domain to bind phosphoenolpyruvate in addition to the kinase-1 and kinase-2 motifs to bind guanosine triphosphate (GTP) and Mg 2+, respectively, specific for all PEPCKs...

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Bibliographic Details
Published in:FEBS letters 2004-03, Vol.561 (1), p.202-206
Main Authors: Schein, Vanessa, Waché, Yann, Etges, Rodrigo, Kucharski, Luiz Carlos, van Wormhoudt, Alain, Da Silva, Roselis S.M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Binding Sites
Brachyura
Chasmagnathus granulata
Crab muscle
Decapoda
Enzyme Induction
Gene expression
Gluconeogenesis
Hyperosmotic shock
Male
Molecular cloning
Molecular Sequence Data
Muscle, Skeletal - metabolism
Osmotic Pressure
Phosphoenolpyruvate carboxykinase
Phosphoenolpyruvate Carboxykinase (GTP) - biosynthesis
Phosphoenolpyruvate Carboxykinase (GTP) - genetics
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Sequence Alignment
Sequence Analysis, DNA
Tissue Distribution
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Summary:Chasmagnathus granulata phosphoenolpyruvate carboxykinase (PEPCK) cDNA from jaw muscle was cloned and sequenced, showing a specific domain to bind phosphoenolpyruvate in addition to the kinase-1 and kinase-2 motifs to bind guanosine triphosphate (GTP) and Mg 2+, respectively, specific for all PEPCKs. In the kinase-1 motifs the GK was changed to RK. The first 19 amino acids of the putative enzyme contain hydrophobic amino acids and hydroxylated residues specific to a mitochondrial type signal. The PEPCK is expressed in hepatopancreas, muscles, nervous system, heart, and gills. Hyperosmotic stress for 24 h increased the PEPCK mRNA level, gluconeogenic and PEPCK activities in muscle.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(04)00162-0