Compound K induces expression of hyaluronan synthase 2 gene in transformed human keratinocytes and increases hyaluronan in hairless mouse skin

Ginsenosides, the major active ingredients of ginseng, have a variety of biomedical efficacies such as anti-aging, anti-oxidation, and anti-inflammatory activities. To understand the effects of compound K (20- O-β- d-glucopyranosyl-20(S)-protopanaxadiol), one of the major metabolites of ginsenosides...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-04, Vol.316 (2), p.348-355
Main Authors: Kim, Sujong, Kang, Byung Young, Cho, Si Yong, Sung, Dae Suk, Chang, Hui Kyung, Yeom, Myung Hun, Kim, Duk Hee, Sim, Young Chul, Lee, Yong Sung
Format: Article
Language:English
Subjects:
Animals
Araliaceae
cDNA microarray
Cell Line
Cell Line, Transformed
Compound K
Epidermis - anatomy & histology
Epidermis - drug effects
Gene Expression - drug effects
Gene Expression Profiling
Ginsenosides
Ginsenosides - chemistry
Ginsenosides - pharmacology
Glucuronosyltransferase - biosynthesis
Glucuronosyltransferase - genetics
HaCaT cell
Humans
Hyaluronan synthase 2
Hyaluronan Synthases
Hyaluronic Acid - biosynthesis
Keratinocytes - cytology
Keratinocytes - metabolism
Mice
Mice, Hairless
Panax ginseng
Skin
Skin - anatomy & histology
Skin - metabolism
Up-Regulation
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Summary:Ginsenosides, the major active ingredients of ginseng, have a variety of biomedical efficacies such as anti-aging, anti-oxidation, and anti-inflammatory activities. To understand the effects of compound K (20- O-β- d-glucopyranosyl-20(S)-protopanaxadiol), one of the major metabolites of ginsenosides, on the skin, we assessed the expression levels of about 100 transcripts in compound K-treated HaCaT cells using cDNA microarray analysis. One gene up-regulated by compound K was hyaluronan synthase2 (HAS2). Semi-quantitative RT-PCR showed that compound K increased HAS2 mRNA in time- and dose-dependent manners. ELISA and immunocytochemistry using hyaluronan (HA)-binding protein showed that compound K effectively increased HA production in HaCaT cells. Finally, treatment of compound K on hairless mouse skin increased the amount of HA in the epidermis and papillary dermis. Our study suggests that topical application of compound K might prevent or improve the deteriorations, such as xerosis and wrinkles, partly ascribed to the age-dependent decrease of the HA content in human skin.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.02.046