Functional Characterization of Serotonin Receptors in Rat Isolated Aorta

Interactions of serotonin (5-HT) with different specific 5-HT receptors that can coexist in the same blood vessel sometimes generate opposite effects. The aim of this study was to characterize the functional role of previously described mRNAs for 5-HT receptors in the rat aorta (5-HT2A, 5-HT2B, 5-HT...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2002, Vol.25(5), pp.584-590
Main Authors: Villazón, María, Padín, Juan Fernando, Cadavid, María Isabel, Enguix, María José, Tristán, Helena, Orallo, Francisco, Loza, María Isabel
Format: Article
Language:English
Subjects:
5-HT receptor
Algorithms
Animals
Aorta, Thoracic - drug effects
Aorta, Thoracic - metabolism
Biological and medical sciences
Blood vessels and receptors
Cyproheptadine - pharmacology
Dinoprost - metabolism
endothelium
Fundamental and applied biological sciences. Psychology
Ketanserin - pharmacology
Male
rat aorta
Rats
Rats, Sprague-Dawley
Receptor, Serotonin, 5-HT2A
Receptors, Serotonin - drug effects
Receptors, Serotonin - metabolism
Reverse Transcriptase Polymerase Chain Reaction
serotonin
Serotonin Antagonists - pharmacology
Vertebrates: cardiovascular system
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Summary:Interactions of serotonin (5-HT) with different specific 5-HT receptors that can coexist in the same blood vessel sometimes generate opposite effects. The aim of this study was to characterize the functional role of previously described mRNAs for 5-HT receptors in the rat aorta (5-HT2A, 5-HT2B, 5-HT1B, 5-HT7) as well as to study the known 5-HT2A receptor-mediated constrictor response and investigate the influences of endothelium and preconstriction on the tissue in that response. A slight endothelium- and concentration-dependent relaxant effect was observed for 5-HT in aorta precontracted with either 5 μM phenylephrine (PE) or 1 μM prostaglandin F2α (PGF2α) in the presence of 0.3 μM ketanserin. EC50 values for 5-HT and α-methyl-5-HT relaxant responses after PE were 43.10±4.00 and 57.11±8.01 nM, respectively. pKB values for antagonists cyproheptadine and rauwolscine were 8.92±0.22 and 7.15±0.12, respectively. In nonprecontracted tissues, the contractile potency of 5-HT was higher in the absence of endothelium (EC50, μM): 2.60±0.28 and 4.12±0.21 in the absence and in the presence of endothelium, respectively. The differences were statistically significant (p
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.25.584