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Identification and immunochemical location of UMP kinase from Bacillus subtilis
Phosphorylation of CMP and UMP is accomplished in Bacillus subtilis, as in Escherichia coli, by two different enzymes exhibiting characteristic structural and catalytic properties. UMP kinase from B. subtilis is an oligomer whose activity is strictly dependent on GTP. The B. subtilis enzyme is unsta...
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| Published in: | Current microbiology 2004-01, Vol.48 (1), p.62-67 |
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| container_end_page | 67 |
| container_issue | 1 |
| container_start_page | 62 |
| container_title | Current microbiology |
| container_volume | 48 |
| creator | Gagyi, Cristina Ionescu, Mihaela Gounon, Pierre Sakamoto, Hiroshi Rousselle, Jean-Claude Laurent-Winter, Christine |
| description | Phosphorylation of CMP and UMP is accomplished in Bacillus subtilis, as in Escherichia coli, by two different enzymes exhibiting characteristic structural and catalytic properties. UMP kinase from B. subtilis is an oligomer whose activity is strictly dependent on GTP. The B. subtilis enzyme is unstable in the absence of UTP, which acts as an allosteric inhibitor. Antibodies raised against recombinant B. subtilis UMP kinase recognized the protein both in soluble extract and in immunoelectron microscopy. UMP kinase from B. subtilis has a peripheral distribution which is related most probably to its role in the synthesis of membrane sugar components and its putative role in cell division. |
| doi_str_mv | 10.1007/s00284-003-4117-2 |
| format | article |
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UMP kinase from B. subtilis is an oligomer whose activity is strictly dependent on GTP. The B. subtilis enzyme is unstable in the absence of UTP, which acts as an allosteric inhibitor. Antibodies raised against recombinant B. subtilis UMP kinase recognized the protein both in soluble extract and in immunoelectron microscopy. 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| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 2004-01, Vol.48 (1), p.62-67 |
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| language | eng |
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| source | Springer Nature |
| subjects | Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - ultrastructure Bacteria Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Bacteriology Blotting, Western DNA, Bacterial - chemistry DNA, Bacterial - genetics E coli Electrophoresis, Gel, Two-Dimensional Guanosine Triphosphate - metabolism Immunohistochemistry Microscopy, Immunoelectron Nucleoside-Phosphate Kinase - isolation & purification Nucleoside-Phosphate Kinase - metabolism Polymerase Chain Reaction |
| title | Identification and immunochemical location of UMP kinase from Bacillus subtilis |
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