Sea urchin neural alpha2 tubulin gene: isolation and promoter analysis

Expression of Talpha2 gene, during sea urchin Paracentrotus lividus development, is spatially and temporally regulated. In order to characterize this gene, we isolated the relevant genomic sequences and scanned the isolated 5'-flanking region in searching for cis-regulatory elements required fo...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-04, Vol.316 (2), p.446-453
Main Authors: Costa, S, Ragusa, M A, Drago, G, Casano, C, Alaimo, G, Guida, N, Gianguzza, F
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cloning, Molecular
Gene Components
Genes
Molecular Sequence Data
Neurons - chemistry
Promoter Regions, Genetic
Sea Urchins - embryology
Sea Urchins - genetics
Sea Urchins - metabolism
Transcription Initiation Site
Transcriptional Activation
Tubulin - genetics
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Summary:Expression of Talpha2 gene, during sea urchin Paracentrotus lividus development, is spatially and temporally regulated. In order to characterize this gene, we isolated the relevant genomic sequences and scanned the isolated 5'-flanking region in searching for cis-regulatory elements required for proper expression. Gel mobility shift and footprinting assays, as well as reporter gene (CAT and beta-gal) expression assays, were used to address cis-regulatory elements involved in regulation. Here we report that an upstream 5'-flanking fragment of PlTalpha2 gene drives temporal expression of reporter genes congruent with that of endogenous Talpha2 gene. The fragment contains cis-elements able to bind nuclear proteins from the gastrula stage (at which the Talpha2 gene is expressed) whose sequences could be consistent with the consensus sequences for transcription factors present in data bank.
ISSN:0006-291X