Effects of high density lipoprotein containing high or low β-carotene concentrations on progesterone production and β-carotene uptake and depletion by bovine luteal cells

Luteal cells were isolated from mid-luteal heifer ovaries by collagenase digestion. Cells were cultured with DMEM/Ham's F12 medium in serum pre-treated plastic culture dishes for periods of up to 11 days. As β-carotene is almost completely insoluble in all polar solvents, it was added to cultur...

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Bibliographic Details
Published in:Animal reproduction science 2000-09, Vol.62 (4), p.253-263
Main Authors: Arikan, Ş, Rodway, R.G
Format: Article
Language:English
Subjects:
Animals
beta Carotene - physiology
Bucladesine - pharmacology
Cattle - physiology
Cattle-feeding and nutrition
Chromatography, High Pressure Liquid - veterinary
Dimethyl Sulfoxide - pharmacology
Female
Furans - pharmacology
Lipoprotein
Lipoproteins, HDL - physiology
Luteal cells
Luteal Cells - chemistry
Luteal Cells - physiology
Luteinizing Hormone - physiology
Male
Progesterone
Progesterone - analysis
Progesterone - biosynthesis
β-Carotene
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Summary:Luteal cells were isolated from mid-luteal heifer ovaries by collagenase digestion. Cells were cultured with DMEM/Ham's F12 medium in serum pre-treated plastic culture dishes for periods of up to 11 days. As β-carotene is almost completely insoluble in all polar solvents, it was added to cultures in either dimethyl sulphoxide (DMSO), tetrahydrofuran (THF) or as high-density lipoprotein (HDL) containing high or low β-carotene concentrations. Medium was replaced after 24 h, thereafter medium was changed every 48 h. Treatment of cells with DMSO alone or with β-carotene (5 μmol/l) in DMSO both resulted in significant ( P
ISSN:0378-4320
1873-2232
DOI:10.1016/S0378-4320(00)00122-6