MMP-2 and MMP-9 Increase the Neurite-Promoting Potential of Schwann Cell Basal Laminae and Are Upregulated in Degenerated Nerve

Compared to degenerated nerves, the ability of normal adult peripheral nerve to support axonal regeneration is poor and may be attributed to the inhibition of endoneurial laminin by chondroitin sulfate proteoglycan (CSPG). In cryoculture assays, neuritic growth of neonatal and adult peripheral neuro...

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Published in:Molecular and cellular neuroscience 2000-08, Vol.16 (2), p.157-167
Main Authors: Ferguson, Toby A., Muir, David
Format: Article
Language:English
Subjects:
Age Factors
Animals
Animals, Newborn
Cells, Cultured
Ganglia, Spinal - cytology
Hyaluronic Acid - metabolism
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 3 - metabolism
Matrix Metalloproteinase 9 - metabolism
Mice
Nerve Crush
Nerve Regeneration - physiology
Neurites - enzymology
Neurons - cytology
Peripheral Nerves - enzymology
Rats
Rats, Sprague-Dawley
Schwann Cells - enzymology
Sciatic Nerve - cytology
Sciatic Nerve - injuries
Sciatic Nerve - physiology
Wallerian Degeneration - metabolism
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Summary:Compared to degenerated nerves, the ability of normal adult peripheral nerve to support axonal regeneration is poor and may be attributed to the inhibition of endoneurial laminin by chondroitin sulfate proteoglycan (CSPG). In cryoculture assays, neuritic growth of neonatal and adult peripheral neurons was increased on sections of normal nerve by pretreatment with CSPG-degrading enzymes, including the matrix metalloproteinases MMP-2 and MMP-9. Axonal regeneration is known to occur within the Schwann cell basal laminae of degenerated nerve. Similarly, deconvolution microscopy revealed that neuritic growth on nerve tissue sections occurred principally on the lumenal surface of enzymatically modified basal laminae. Compared to normal nerve, there was a marked increase in the neurite-promoting activity of the degenerated nerve, and this activity was not increased significantly by subsequent MMP treatment. Additionally, the expression and activation of MMP-2 and MMP-9 were elevated in degenerated nerve, suggesting that degradation of inhibitory CSPG by the MMPs contributes to the growth-promoting properties of degenerated nerve.
ISSN:1044-7431
1095-9327
DOI:10.1006/mcne.2000.0859