Extracellular polysaccharide composition of Azospirillum brasilense and its relation with cell aggregation

Abstract The exopolysaccharide (EPS) and capsular polysaccharide (CPS) composition of four Azospirillum brasilense strains differing in their aggregation capacity was analyzed by high performance anion exchange chromatography. When growing the different strains in an aggregation inducing medium cont...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-08, Vol.189 (2), p.259-264
Main Authors: Burdman, S., Jurkevitch, E., Soria-Díaz, María E., Serrano, Antonio M.Gil, Okon, Y.
Format: Article
Language:English
Subjects:
Agglomeration
Anion exchange
Anion exchanging
Arabinose
Azospirillum brasilense
Azospirillum brasilense - cytology
Azospirillum brasilense - metabolism
Bacterial Adhesion
Bacteriology
Bioassays
Biological and medical sciences
Capsular polysaccharide
Capsular polysaccharides
Cell aggregation
Composition
Exopolysaccharide
Exopolysaccharides
Extracellular Matrix - metabolism
Flocculation
Fundamental and applied biological sciences. Psychology
Galactose
Microbiology
Monosaccharides
Morphology, structure, chemical composition
Nitrogen
Polysaccharides
Polysaccharides - metabolism
Saccharides
Strains (organisms)
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Summary:Abstract The exopolysaccharide (EPS) and capsular polysaccharide (CPS) composition of four Azospirillum brasilense strains differing in their aggregation capacity was analyzed by high performance anion exchange chromatography. When growing the different strains in an aggregation inducing medium containing a high carbon:nitrogen (C:N) ratio, both EPS and CPS showed a positive correlation between aggregation and the relative amount of arabinose. Arabinose was not detected in polysaccharides from Sp72002, a pleiotrophic Tn5 mutant strain impaired in aggregation. Arabinose was also not detected in extracellular polysaccharides of bacteria grown in a low C:N ratio, non-inducing aggregation medium, with exception for a relatively small amount found in the CPS of FAJ0204, a super-aggregating mutant strain. The only monosaccharides able to significantly inhibit aggregation at low sugar concentration when tested in a bioassay were arabinose (at a higher extent) and galactose. The possibility that residues of arabinose present in the extracellular polysaccharides are involved in the aggregation of A. brasilense is discussed.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09240.x