Pilot studies on the p53 gene in nipple aspirate fluid from patients with breast cancer

Nipple Aspirate Fluid (NAF) from patients with breast cancer is a potential source of exfoliated tumour material amenable to molecular biological study, but few such data have been reported. In this study we demonstrate that polymerase chain reaction (PCR) amplification of p53 gene DNA is achievable...

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Bibliographic Details
Published in:Breast cancer research and treatment 2000-05, Vol.61 (2), p.139-143
Main Authors: PHILLIPS, H. A, HOWARD, G. C. W, MILLER, W. R
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Body Fluids - chemistry
Breast cancer
Breast Neoplasms - chemistry
Breast Neoplasms - genetics
Cancer research
Cancer therapies
Cohort Studies
DNA Mutational Analysis
DNA, Neoplasm - genetics
DNA, Neoplasm - isolation & purification
Female
Genes, p53
Gynecology. Andrology. Obstetrics
Humans
Mammary gland diseases
Medical sciences
Neoplasm Proteins - genetics
Nipples
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Reagent Kits, Diagnostic
Sensitivity and Specificity
Tumors
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Summary:Nipple Aspirate Fluid (NAF) from patients with breast cancer is a potential source of exfoliated tumour material amenable to molecular biological study, but few such data have been reported. In this study we demonstrate that polymerase chain reaction (PCR) amplification of p53 gene DNA is achievable in a proportion of NAF samples from breast cancer patients. Subsequently four NAF samples from patients whose primary tumours were identified as having a defined p53 mutation were studied by single stranded conformational polymorphism analysis (SSCP). Two samples yielded PCR product indistinguishable from wild type and two yielded no product. Whilst no cancer-related genetic mutations were demonstrated in NAF samples, further study is warranted.
ISSN:0167-6806
1573-7217
DOI:10.1023/A:1006487315587