Antigenic secreted proteins from Haemophilus paragallinarum. A 110-kDa putative RTX protein

• Published in: FEMS microbiology letters 2004-03, Vol.232 (1), p.83-87
• Main Authors: Mena-Rojas, Erika, Vázquez Cruz, Candelario, Vaca Pacheco, Sergio, Garcı́a González, Octavio, Pérez-Márquez, Vı́ctor M, Pérez-Méndez, Alma, Ibarra-Caballero, Jorge, de la Garza, Mireya, Zenteno, Edgar, Negrete-Abascal, Erasmo
• Format: Article
• Language: English
• Subjects: Actinobacillus Infections - immunology; Actinobacillus Infections - microbiology; Actinobacillus Infections - veterinary; Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae - immunology; Animals; Antibodies, Bacterial - blood; Antigens, Bacterial - immunology; Antigens, Bacterial - metabolism; Apx protein; Bacterial Proteins - immunology; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Blotting, Western; Chickens - immunology; Cross Reactions; Culture Media; Exotoxins - immunology; Fundamental and applied biological sciences. Psychology; Haemophilus Infections - immunology; Haemophilus Infections - microbiology; Haemophilus Infections - veterinary; Haemophilus paragallinarum; Haemophilus paragallinarum - growth & development; Haemophilus paragallinarum - immunology; Haemophilus paragallinarum - metabolism; Immune cross-reactivity; Infectious coryza; Mannheimia haemolytica - immunology; Microbiology; Miscellaneous; Molecular Weight; Pasteurellaceae Infections - immunology; Pasteurellaceae Infections - veterinary; Poultry Diseases - immunology; Rabbits - immunology; RTX protein; Secreted antigen; Swine - immunology; Swine Diseases - immunology
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(04)00036-9

Haemophilus paragallinarum is the causal agent of infectious coryza, an economically important disease for the poultry industry. This bacterium secreted proteins of 25–110 kDa during its growth in brain heart infusion, tryptic soy broth, or Luria–Bertani glucose phosphate media, all lacking serum. Some of these proteins were recognized by sera from chickens experimentally infected with H. paragallinarum. A 110-kDa protein was recognized by a serum pool from convalescent-phase pigs naturally infected with Actinobacillus pleuropneumoniae, and also by a rabbit polyclonal serum against Apx I as well as a rabbit serum against Mannheimia haemolytica leukotoxin, suggesting the presence of an RTX-like protein in H. paragallinarum. H. paragallinarum secreted proteins could be important immunogens in the control of infectious coryza.