Identification of a rare point mutation at C-terminus of merozoite surface antigen-1 gene of Plasmodium falciparum in eastern Indian isolates

Merozoite surface antigen-1 (MSA-1) of Plasmodium falciparum is highly immunogenic in human. Several studies suggest that MSA-1 protein is an effective target for a protective immune response. Attempt has been made to find new point mutations by analyzing 244 bp [codon 1655(R) to 1735 (I)] relativel...

Full description

Saved in:
Bibliographic Details
Published in:Experimental parasitology 2004-01, Vol.106 (1), p.45-49
Main Authors: Raj, Dipak Kumar, Das, Bibhu Ranjan, Dash, A.P., Supakar, Prakash C.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
DNA, Protozoan - chemistry
Fundamental and applied biological sciences. Psychology
Humans
Life cycle. Host-agent relationship. Pathogenesis
Merozoite Surface Protein 1 - genetics
Nucleic Acid Hybridization
Nucleotide Mapping
Oligonucleotide Probes - chemistry
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - immunology
Point Mutation
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Protozoa
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Merozoite surface antigen-1 (MSA-1) of Plasmodium falciparum is highly immunogenic in human. Several studies suggest that MSA-1 protein is an effective target for a protective immune response. Attempt has been made to find new point mutations by analyzing 244 bp [codon 1655(R) to 1735 (I)] relatively conserved C-terminus region of MSA-1 gene in 125 isolates. This region contains two EGF like domains, which are involved in generating protective immune response in human. Point mutations in this region are very much important in view of vaccine development. Searching of mutational hot spots in MSA-1 protein by sequencing method in a representative number of isolates is quite critical and expensive. Therefore, in this study slot blot and PCR-SSCP method have been used to find out new mutations in the individual isolates showing alterations in the mobility of DNA fragment. Sequencing of the altered bands from the SSCP gel shows a rare non-synonymous point mutation in 7 (5.6%) of the 125 isolates at amino acid position 1704 of MSA-1 gene where isoleucine is replaced by valine. Index Descriptors and Abbreviations: Plasmodium falciparum; merozoite surface antigen-1 (MSA-1) gene; gene mutation and recombinant vaccine; SSCP, single strand conformational polymorphism; EGF, epidermal growth factor
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2004.01.008