Ultrasound-guided left-ventricular catheterization: a novel method of whole mouse perfusion for microimaging

We describe a novel technique to perform whole-body perfusion fixation in mice with specific relevance to micro-imaging. With the guidance of high-frequency ultrasound imaging, we were able to perfuse fixative and contrast agents via a catheter inserted into the left ventricle, and therefore preserv...

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Bibliographic Details
Published in:Laboratory investigation 2004-03, Vol.84 (3), p.385-389
Main Authors: Zhou, Yu-Qing, Davidson, Lorinda, Henkelman, R Mark, Nieman, Brian J, Foster, F Stuart, Yu, Lisa X, Chen, X Josette
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Cardiac Catheterization - methods
Female
Fundamental and applied biological sciences. Psychology
Heart Ventricles - diagnostic imaging
Investigative techniques, diagnostic techniques (general aspects)
Laboratory Medicine
Magnetic Resonance Imaging - methods
Male
Medical sciences
Medicine
Medicine & Public Health
Mice
Mice, Inbred C57BL
micro-CT
Models, Anatomic
MRI
Pathology
Perfusion - methods
perfusion–fixation
technical-report
Tissue Fixation - methods
Tomography, X-Ray Computed - methods
Ultrasonography
ultrasound biomicroscopy
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Summary:We describe a novel technique to perform whole-body perfusion fixation in mice with specific relevance to micro-imaging. With the guidance of high-frequency ultrasound imaging, we were able to perfuse fixative and contrast agents via a catheter inserted into the left ventricle, and therefore preserved the integrity of the chest and abdominal cavity. In this preliminary study, our success rate over 15 animals was 73%. We demonstrate applications of this technique for magnetic resonance imaging and micro-CT, but we expect that this method can be generally applied to whole-body perfusions of other small animals in which the intact body is necessary.
ISSN:0023-6837
1530-0307
DOI:10.1038/labinvest.3700038