Commercial and laboratory extracts from artichoke leaves: estimation of caffeoyl esters and flavonoidic compounds content

Artichoke leaf extracts are widely used alone or in association with other herbs for embittering alcoholic and soft drinks and to prepare herbal teas or herbal medicinal products. Despite this wide diffusion, the European Pharmacopoeia does not report an official method for the determination of the...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2004-02, Vol.34 (2), p.349-357
Main Authors: Mulinacci, N, Prucher, D, Peruzzi, M, Romani, A, Pinelli, P, Giaccherini, C, Vincieri, F.F
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Artichoke leaf extract
Biological and medical sciences
Caffeic acids
Caffeic Acids - analysis
Caffeic Acids - chemistry
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Cynara scolymus
Esters
Flavonoids
Flavonoids - analysis
Flavonoids - chemistry
Fundamental and applied biological sciences. Psychology
General pharmacology
HPLC/DAD
HPLC/MS
Medical sciences
Pharmacology. Drug treatments
Plant Extracts - analysis
Plant Extracts - chemistry
Plant Leaves
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Summary:Artichoke leaf extracts are widely used alone or in association with other herbs for embittering alcoholic and soft drinks and to prepare herbal teas or herbal medicinal products. Despite this wide diffusion, the European Pharmacopoeia does not report an official method for the determination of the active principles of artichoke leaf extracts. This work reports a quali-quantitative determination by HPLC/DAD and HPLC/MS techniques of both cynnamic acids and flavonoids present in some artichoke leaf commercial extracts (Com) compared with two different laboratory extracts (Lab). Most of the commercial extracts showed a similar quali-quantitative pattern with a single exception having five–six times higher value. The quantitative data from the Italian Pharmacopoeia(IP) official method does not evaluate the flavonoidic fraction and showed an overestimation of the caffeoyl esters with respect to the HPLC/DAD results. The proposed HPLC/DAD method was able to completely characterize and quantify this matrix and represents a contribution to better quality control of these herbal extracts.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(03)00552-1