Recent developments for SPIE-IA, a new sandwich immunoassay format for very small molecules

Recent publications describing new elegant approaches to assay small analytes using noncompetitive format were briefly reviewed. Among these methods, we have developed a new protocol, named SPIE-IA, which involves a cross-linking step achieved using chemical hombifunctional reagents, UV irradiation...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2004-03, Vol.34 (4), p.737-752
Main Authors: Volland, Hervé, Pradelles, Philippe, Taran, Frédéric, Buscarlet, Laure, Creminon, Christophe
Format: Article
Language:English
Subjects:
Antioxidants - analysis
Antioxidants - pharmacology
Cell culture
Cross-Linking Reagents - analysis
Epitopes - analysis
Epitopes - immunology
France
Free radicals
Free Radicals - analysis
Immune complex
Immunoassay - methods
Immunoassay - trends
Irradiation
Models, Immunological
Noncompetitive assays
Oxidant stress
Small analytes
Ultraviolet Rays - adverse effects
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Summary:Recent publications describing new elegant approaches to assay small analytes using noncompetitive format were briefly reviewed. Among these methods, we have developed a new protocol, named SPIE-IA, which involves a cross-linking step achieved using chemical hombifunctional reagents, UV irradiation or free radicals. This new method proved to be useful to detect naturally occurring analyte/antibody complexes or to protect the analytes against degradation by peptidases. On the other hand, SPIE-IA could allow to study the adverse biological effects of UV and some aspects of free radical chemistry or to evaluate the antioxidant activity of molecules.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(03)00560-0