Sex- and stage-related sensitivity of Schistosoma mansoni to in vivo and in vitro praziquantel treatment

The efficacy of praziquantel against a Puerto Rican strain of Schistosoma mansoni was assessed using both in vivo and in vitro approach. The drug effective dose (50%) in the infected mouse model was about 30 times higher when determined against 28-day-old infections than against 7-week-old parasites...

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Bibliographic Details
Published in:International journal for parasitology 2004-03, Vol.34 (4), p.527-533
Main Authors: Pica-Mattoccia, Livia, Cioli, Donato
Format: Article
Language:English
Subjects:
Animals
Anthelmintics - therapeutic use
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiparasitic agents
Biological and medical sciences
Biomphalaria - parasitology
Drug sensitivity
Female
Fundamental and applied biological sciences. Psychology
Gender Identity
Host parasite relation
pathogenicity
Immature schistosomes
In vitro culture
Invertebrates
Life Cycle Stages
Male
Medical sciences
Mice
Mice, Inbred Strains
Nemathelminthia. Plathelmintha
Parasitic Sensitivity Tests
Pharmacology. Drug treatments
Praziquantel
Praziquantel - therapeutic use
Puerto Rico
Schistosoma mansoni
Schistosoma mansoni - drug effects
Schistosoma mansoni - physiology
Schistosomiasis - drug therapy
Sex differences
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Summary:The efficacy of praziquantel against a Puerto Rican strain of Schistosoma mansoni was assessed using both in vivo and in vitro approach. The drug effective dose (50%) in the infected mouse model was about 30 times higher when determined against 28-day-old infections than against 7-week-old parasites. Single-sex female infections were also largely refractory to treatment and single-sex male infections moderately refractory, in comparison with bisexual infections. The in vitro approach consisted of overnight exposure of parasite cultures to various drug concentrations, followed by several days of culture in drug-free medium. In vitro results confirmed in vivo data and allowed for the observation of schistosome morphological phenomena after praziquantel exposure. Early worm contraction was observed in all cases, even after exposure to sub-lethal concentrations of praziquantel or upon exposure of the largely refractory 28-day-old schistosomes. In these instances, however, worms resumed movements and normal shape upon drug removal and were able to survive. The inference of these observations on the clinical use of praziquantel and on its mechanism of action is discussed.
ISSN:0020-7519
1879-0135
DOI:10.1016/j.ijpara.2003.12.003