Effects of anti-GLUT antibodies on glucose transport into human erythrocyte ghosts

We have studied the effects of anti-GLUT1 antibodies on the uptake of glucose into erythrocytes. Glucose transport into human erythrocyte ghosts was measured directly using 3H-2-deoxy-glucose, or indirectly by monitoring associated volume changes using light scattering. The uptake of glucose was sig...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2004-05, Vol.62 (2), p.195-198
Main Authors: Afzal, I., Browning, J.A., Drew, C., Ellory, J.C., Naftalin, R.J., Wilkins, R.J.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - pharmacology
Antibody
Antibody Specificity
Cell Size
Epitopes
Erythrocyte
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - metabolism
Ghost
Glucose - metabolism
Glucose Transporter Type 1
GLUT1
Humans
Kinetics
Monosaccharide Transport Proteins - immunology
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Summary:We have studied the effects of anti-GLUT1 antibodies on the uptake of glucose into erythrocytes. Glucose transport into human erythrocyte ghosts was measured directly using 3H-2-deoxy-glucose, or indirectly by monitoring associated volume changes using light scattering. The uptake of glucose was significantly inhibited in ghosts resealed in solutions containing specific antibodies against GLUT1. Such an effect was not observed when an antibody against the oestrogen receptor, lacking specificity towards GLUT1, was employed instead. The antibodies were also without effect on the efflux of preloaded glucose from erythrocyte ghosts. The demonstration that anti-GLUT antibodies can inhibit glucose uptake is support for the hypothesis that they exaggerate the cytoplasmic barrier to glucose uptake created by endofacial segments of GLUT1.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2003.07.007