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WBC subset analysis of WBC-reduced platelet components
BACKGROUND: WBC‐reduced platelet components may be prepared by filtration or apheresis processing. Both methods have previously been shown to result in a residual total WBC content
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| Published in: | Transfusion (Philadelphia, Pa.) Pa.), 2000-07, Vol.40 (7), p.771-780 |
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| cited_by | cdi_FETCH-LOGICAL-c4981-972f1176a665d1a60c00f89980d7b50da352eb5b92d4331999432176c4845e703 |
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| cites | cdi_FETCH-LOGICAL-c4981-972f1176a665d1a60c00f89980d7b50da352eb5b92d4331999432176c4845e703 |
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| container_title | Transfusion (Philadelphia, Pa.) |
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| creator | Triulzi, D.J. Meyer, E.M. Donnenberg, A.D. |
| description | BACKGROUND: WBC‐reduced platelet components may be prepared by filtration or apheresis processing. Both methods have previously been shown to result in a residual total WBC content |
| doi_str_mv | 10.1046/j.1537-2995.2000.40070771.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71747605</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71747605</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4981-972f1176a665d1a60c00f89980d7b50da352eb5b92d4331999432176c4845e703</originalsourceid><addsrcrecordid>eNqVkE1r20AQhpfS0DhO_kIRtOQmZfZbS0-tW7sJxgnBIcdltVqBXFlytRKx_31WyHF7zWmZnWfeGR6EvmBIMDBxs0kwpzImSvGEAEDCACRIiZP9BzQ59T6iCQDDMcaUnKML7zeBJQrwJ3SOQREmgE6QeP4xi3yfeddFpjbVwZc-aooofMety3vr8mhXmc5VAbDNdtfUru78JTorTOXd1fGdoqf5r_Xsd7y8X9zOvi9jy1SKYyVJgbEURgieYyPAAhSpUinkMuOQG8qJy3imSM4oxUopRkngLUsZdxLoFF2Pubu2-ds73-lt6a2rKlO7pvdaYsmkAB7AbyNo28b71hV615Zb0x40Bj1o0xs9qNGDGj1o02_a9D5Mfz6u6bOty_-bHT0F4OsRMN6aqmhNbUv_j2MsxA7n_hyxl7Jyh_ecoNeP87cqxMRjTOk7tz_FmPaPFpJKrp9XC71cPzyo9G6lV_QVa86WEA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71747605</pqid></control><display><type>article</type><title>WBC subset analysis of WBC-reduced platelet components</title><source>Wiley</source><creator>Triulzi, D.J. ; Meyer, E.M. ; Donnenberg, A.D.</creator><creatorcontrib>Triulzi, D.J. ; Meyer, E.M. ; Donnenberg, A.D.</creatorcontrib><description>BACKGROUND: WBC‐reduced platelet components may be prepared by filtration or apheresis processing. Both methods have previously been shown to result in a residual total WBC content <5 × 106 per component. However, there may be differences in the efficacy of these techniques for removing certain WBC subsets.
STUDY DESIGN AND METHODS: Two multiparameter flow cytometric assays were developed and validated to perform WBC analysis on WBC‐reduced platelets collected with two apheresis instruments (Amicus and COBE Spectra) and on 6 units of filtered pooled random‐donor platelet concentrates.
RESULTS: All components contained <1 × 105 WBCs. The COBE Spectra and Amicus apheresis platelet components contained more WBCs than did filtered pooled platelets (p<0.05). Lymphocytes (T and B), monocytes, and granulocytes were identified in all components. Granulocyte content was lowest in the Amicus components and filtered pools. Monocytes were lowest in filtered pools. Amicus platelet components had fewer granulocytes and monocytes than the COBE Spectra platelets. Amicus and COBE Spectra components contained more lymphocytes than the filtered pools.
CONCLUSION: Multiparameter flow cytometry can be used to quantify and characterize WBCs in WBC‐reduced platelet components. WBC reduction by filtration or apheresis was highly effective. WBCs from each subset were identified in all components. Although filtered pools had the lowest numbers of WBCs, the very low numbers observed in all components suggests that the absolute quantitative differences in WBC subset content are of questionable clinical significance.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1046/j.1537-2995.2000.40070771.x</identifier><identifier>PMID: 10924603</identifier><identifier>CODEN: TRANAT</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Inc</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Blood Cell Count ; Blood Platelets - cytology ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; BSG-C = buffered saline glucose-citrate ; cRBC(s) = chicken RBC(s) ; Flow Cytometry - methods ; Humans ; Medical sciences ; PI = propidium iodide ; Plateletpheresis - methods ; RDP(s) = random-donor platelet concentrate(s) ; SDP(s) = single-donor apheresis platelet(s) ; Sensitivity and Specificity ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Transfusion (Philadelphia, Pa.), 2000-07, Vol.40 (7), p.771-780</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4981-972f1176a665d1a60c00f89980d7b50da352eb5b92d4331999432176c4845e703</citedby><cites>FETCH-LOGICAL-c4981-972f1176a665d1a60c00f89980d7b50da352eb5b92d4331999432176c4845e703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1449950$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10924603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Triulzi, D.J.</creatorcontrib><creatorcontrib>Meyer, E.M.</creatorcontrib><creatorcontrib>Donnenberg, A.D.</creatorcontrib><title>WBC subset analysis of WBC-reduced platelet components</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>BACKGROUND: WBC‐reduced platelet components may be prepared by filtration or apheresis processing. Both methods have previously been shown to result in a residual total WBC content <5 × 106 per component. However, there may be differences in the efficacy of these techniques for removing certain WBC subsets.
STUDY DESIGN AND METHODS: Two multiparameter flow cytometric assays were developed and validated to perform WBC analysis on WBC‐reduced platelets collected with two apheresis instruments (Amicus and COBE Spectra) and on 6 units of filtered pooled random‐donor platelet concentrates.
RESULTS: All components contained <1 × 105 WBCs. The COBE Spectra and Amicus apheresis platelet components contained more WBCs than did filtered pooled platelets (p<0.05). Lymphocytes (T and B), monocytes, and granulocytes were identified in all components. Granulocyte content was lowest in the Amicus components and filtered pools. Monocytes were lowest in filtered pools. Amicus platelet components had fewer granulocytes and monocytes than the COBE Spectra platelets. Amicus and COBE Spectra components contained more lymphocytes than the filtered pools.
CONCLUSION: Multiparameter flow cytometry can be used to quantify and characterize WBCs in WBC‐reduced platelet components. WBC reduction by filtration or apheresis was highly effective. WBCs from each subset were identified in all components. Although filtered pools had the lowest numbers of WBCs, the very low numbers observed in all components suggests that the absolute quantitative differences in WBC subset content are of questionable clinical significance.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Blood Cell Count</subject><subject>Blood Platelets - cytology</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>BSG-C = buffered saline glucose-citrate</subject><subject>cRBC(s) = chicken RBC(s)</subject><subject>Flow Cytometry - methods</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>PI = propidium iodide</subject><subject>Plateletpheresis - methods</subject><subject>RDP(s) = random-donor platelet concentrate(s)</subject><subject>SDP(s) = single-donor apheresis platelet(s)</subject><subject>Sensitivity and Specificity</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqVkE1r20AQhpfS0DhO_kIRtOQmZfZbS0-tW7sJxgnBIcdltVqBXFlytRKx_31WyHF7zWmZnWfeGR6EvmBIMDBxs0kwpzImSvGEAEDCACRIiZP9BzQ59T6iCQDDMcaUnKML7zeBJQrwJ3SOQREmgE6QeP4xi3yfeddFpjbVwZc-aooofMety3vr8mhXmc5VAbDNdtfUru78JTorTOXd1fGdoqf5r_Xsd7y8X9zOvi9jy1SKYyVJgbEURgieYyPAAhSpUinkMuOQG8qJy3imSM4oxUopRkngLUsZdxLoFF2Pubu2-ds73-lt6a2rKlO7pvdaYsmkAB7AbyNo28b71hV615Zb0x40Bj1o0xs9qNGDGj1o02_a9D5Mfz6u6bOty_-bHT0F4OsRMN6aqmhNbUv_j2MsxA7n_hyxl7Jyh_ecoNeP87cqxMRjTOk7tz_FmPaPFpJKrp9XC71cPzyo9G6lV_QVa86WEA</recordid><startdate>200007</startdate><enddate>200007</enddate><creator>Triulzi, D.J.</creator><creator>Meyer, E.M.</creator><creator>Donnenberg, A.D.</creator><general>Blackwell Science Inc</general><general>Blackwell Publishing</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200007</creationdate><title>WBC subset analysis of WBC-reduced platelet components</title><author>Triulzi, D.J. ; Meyer, E.M. ; Donnenberg, A.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4981-972f1176a665d1a60c00f89980d7b50da352eb5b92d4331999432176c4845e703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Blood Cell Count</topic><topic>Blood Platelets - cytology</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>BSG-C = buffered saline glucose-citrate</topic><topic>cRBC(s) = chicken RBC(s)</topic><topic>Flow Cytometry - methods</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>PI = propidium iodide</topic><topic>Plateletpheresis - methods</topic><topic>RDP(s) = random-donor platelet concentrate(s)</topic><topic>SDP(s) = single-donor apheresis platelet(s)</topic><topic>Sensitivity and Specificity</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Triulzi, D.J.</creatorcontrib><creatorcontrib>Meyer, E.M.</creatorcontrib><creatorcontrib>Donnenberg, A.D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Triulzi, D.J.</au><au>Meyer, E.M.</au><au>Donnenberg, A.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>WBC subset analysis of WBC-reduced platelet components</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2000-07</date><risdate>2000</risdate><volume>40</volume><issue>7</issue><spage>771</spage><epage>780</epage><pages>771-780</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><coden>TRANAT</coden><abstract>BACKGROUND: WBC‐reduced platelet components may be prepared by filtration or apheresis processing. Both methods have previously been shown to result in a residual total WBC content <5 × 106 per component. However, there may be differences in the efficacy of these techniques for removing certain WBC subsets.
STUDY DESIGN AND METHODS: Two multiparameter flow cytometric assays were developed and validated to perform WBC analysis on WBC‐reduced platelets collected with two apheresis instruments (Amicus and COBE Spectra) and on 6 units of filtered pooled random‐donor platelet concentrates.
RESULTS: All components contained <1 × 105 WBCs. The COBE Spectra and Amicus apheresis platelet components contained more WBCs than did filtered pooled platelets (p<0.05). Lymphocytes (T and B), monocytes, and granulocytes were identified in all components. Granulocyte content was lowest in the Amicus components and filtered pools. Monocytes were lowest in filtered pools. Amicus platelet components had fewer granulocytes and monocytes than the COBE Spectra platelets. Amicus and COBE Spectra components contained more lymphocytes than the filtered pools.
CONCLUSION: Multiparameter flow cytometry can be used to quantify and characterize WBCs in WBC‐reduced platelet components. WBC reduction by filtration or apheresis was highly effective. WBCs from each subset were identified in all components. Although filtered pools had the lowest numbers of WBCs, the very low numbers observed in all components suggests that the absolute quantitative differences in WBC subset content are of questionable clinical significance.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Inc</pub><pmid>10924603</pmid><doi>10.1046/j.1537-2995.2000.40070771.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Wiley |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Blood Cell Count Blood Platelets - cytology Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis BSG-C = buffered saline glucose-citrate cRBC(s) = chicken RBC(s) Flow Cytometry - methods Humans Medical sciences PI = propidium iodide Plateletpheresis - methods RDP(s) = random-donor platelet concentrate(s) SDP(s) = single-donor apheresis platelet(s) Sensitivity and Specificity Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | WBC subset analysis of WBC-reduced platelet components |
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