Differences in regulation of pH(i) in large (>/=10 nuclei) and small (</=5 nuclei) osteoclasts

Osteoclasts are multinucleated cells that resorb bone by extrusion of protons and proteolytic enzymes. They display marked heterogeneity in cell size, shape, and resorptive activity. Because high resorptive activity in vivo is associated with an increase in the average size of osteoclasts in areas o...

Full description

Saved in:
Bibliographic Details
Published in:American Journal of Physiology: Cell Physiology 2000-09, Vol.279 (3), p.C751-C761
Main Authors: Lees, R L, Heersche, J N
Format: Article
Language:English
Subjects:
Acids - pharmacology
Amiloride - pharmacology
Ammonium Chloride - pharmacology
Animals
Anti-Bacterial Agents - pharmacology
Calibration
Cell Size
Cells, Cultured
Chloride Channels - metabolism
Enzyme Inhibitors - pharmacology
Extracellular Space - metabolism
Hydrogen - metabolism
Hydrogen-Ion Concentration
Intracellular Membranes - metabolism
Macrolides
Osteoclasts - cytology
Osteoclasts - drug effects
Osteoclasts - metabolism
Proton-Translocating ATPases - metabolism
Rabbits
Sodium - metabolism
Vacuolar Proton-Translocating ATPases
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Osteoclasts are multinucleated cells that resorb bone by extrusion of protons and proteolytic enzymes. They display marked heterogeneity in cell size, shape, and resorptive activity. Because high resorptive activity in vivo is associated with an increase in the average size of osteoclasts in areas of greater resorption and because of the importance of proton extrusion in resorption, we investigated whether the activity of the bafilomycin A(1)-sensitive vacuolar-type H(+)-ATPase (V-ATPase) and amiloride-sensitive Na(+)/H(+) exchanger differed between large and small osteoclasts. Osteoclasts were obtained from newborn rabbit bones, cultured on glass coverslips, and loaded with the pH-sensitive indicator 2', 7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Intracellular pH (pH(i)) was recorded in single osteoclasts by monitoring fluorescence. Large (>/=10 nuclei) and small (
ISSN:0363-6143
DOI:10.1152/ajpcell.2000.279.3.c751