Separation of coumarins from Archangelica officinalis in high-performance liquid chromatography and thin-layer chromatography systems

Complex, multicomponent mixtures are difficult to separate in a single chromatographic run. Therefore, the possibility to separate twelve coumarins from Archangelica officinalis was studied by combining a HPLC and a TLC system. HPLC optimized by the use of DryLab for Windows software was performed o...

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Bibliographic Details
Published in:Journal of Chromatography A 2000-07, Vol.886 (1), p.75-81
Main Authors: Hawryl, Miroslaw A, Soczewinski, Edward, Dzido, Tadeusz H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer - methods
Coumarins
Coumarins - isolation & purification
General pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plants - chemistry
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Summary:Complex, multicomponent mixtures are difficult to separate in a single chromatographic run. Therefore, the possibility to separate twelve coumarins from Archangelica officinalis was studied by combining a HPLC and a TLC system. HPLC optimized by the use of DryLab for Windows software was performed on RP-18 column and TLC was performed on silica plates. Fractions from the RP column were evaporated, applied on silica plate and developed in non-aqueous solvent. Possibilities of complete separation of investigated coumarins were discussed in RP and NP systems. The result of their complete separation was presented by HPLC chromatograms, DryLab simulated chromatograms and a video scan of TLC plate.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00321-6