Genetic diversity of bluetongue viruses in south east Asia

Bluetongue viruses (BTV) were isolated from sentinel cattle in Malaysia and at two sites in Indonesia. We identified eight serotypes some of which appeared to have a wide distribution throughout this region, while others were only isolated in Malaysia or Australia. Nearly half of the 24 known BTV se...

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Bibliographic Details
Published in:Virus research 2004-05, Vol.101 (2), p.193-201
Main Authors: Pritchard, L.I, Sendow, I, Lunt, R, Hassan, S.H, Kattenbelt, J, Gould, A.R, Daniels, P.W, Eaton, B.T
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Asia, Southeastern - epidemiology
Bluetongue - epidemiology
Bluetongue - virology
Bluetongue virus
Bluetongue virus - genetics
Bluetongue virus - isolation & purification
Bluetongue viruse
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - virology
Evolution, Molecular
Genetic diversity
Genetic Variation
Genotype
Molecular Epidemiology
Molecular Sequence Data
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - isolation & purification
Sentinel Surveillance
Serotyping
South east Asia
Viral Core Proteins - genetics
Viral Nonstructural Proteins - chemistry
Viral Nonstructural Proteins - genetics
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Summary:Bluetongue viruses (BTV) were isolated from sentinel cattle in Malaysia and at two sites in Indonesia. We identified eight serotypes some of which appeared to have a wide distribution throughout this region, while others were only isolated in Malaysia or Australia. Nearly half of the 24 known BTV serotypes have now been identified in Asia. Further, we investigated the genetic diversity of their RNA segments 3 and 10. Using partial nucleotide sequences of the RNA segment 3 (540 bp) which codes for the conserved core protein (VP3), the BTV isolates were found to be unique to the previously defined Australasian topotype and could be further subdivided into four distinct clades or genotypes. Certain of these genotypes appeared to be geographically restricted while others were distributed widely throughout the region. Similarly, the complete nucleotide sequences of the RNA segment 10 (822 bp), coding for the non-structural protein (NS3/3A), were also conserved and grouped into the five genotypes; the BTV isolates could be grouped into three Asian genotypes and two Nth American/Sth African genotypes.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2004.01.004