Infrequent loss of heterozygosity at adenomatous polyposis coli gene locus in Indian oral cancers

We have investigated loss of heterozygosity (LOH) of the adenomatous polyposis coli (APC) tumor suppressor gene using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) in 86 untreated oral cancer patients, using matched oral cancer tissue and corresponding peripheral bl...

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Bibliographic Details
Published in:Cancer letters 2000-09, Vol.157 (2), p.155-160
Main Authors: Tandle, Anita, Sanghavi, Vikram, Saranath, Dhananjaya
Format: Article
Language:English
Subjects:
Adenomatous polyposis coli
Adult
Aged
Alleles
APC gene
Biological and medical sciences
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
DNA, Neoplasm - genetics
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Female
Genes, APC - genetics
Humans
India
Loss of Heterozygosity
Male
Medical sciences
Middle Aged
Mouth Neoplasms - genetics
Mouth Neoplasms - pathology
Neoplasm Staging
Oral cancer
Otorhinolaryngology. Stomatology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Tumors
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Summary:We have investigated loss of heterozygosity (LOH) of the adenomatous polyposis coli (APC) tumor suppressor gene using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) in 86 untreated oral cancer patients, using matched oral cancer tissue and corresponding peripheral blood cell (PBC) DNA samples. PBC from 70 normal healthy individuals, were also analyzed for allelic distribution of APC gene. A 133 bp fragment, spanning exon 11 of the APC gene was amplified, and RsaI digestion of the PCR product defined the alleles as either homozygous 133 bp (Rsa −/−) or 87 and 46 bp (Rsa +/+) fragments, and heterozygous (Rsa +/−) exhibiting the three fragments. Distribution of the three alleles, Rsa −/−, Rsa +/+, and Rsa +/− in the oral cancer patients was observed as 10.5, 51.1 and 38.4%; whereas normal healthy individuals showed 11.4, 37.1 and 51.4%, respectively. In the informative heterozygous (Rsa +/−) oral cancer patients, LOH was infrequent, demonstrated in two of 33 (6%) samples. Thus, the APC gene was infrequently altered by LOH at the polymorphic RsaI locus in exon 11 in the tobacco associated Indian oral cancer, unlike the smoking tobacco/alcohol associated oral cancers from Western countries.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(00)00484-5