Reprimo, a New Candidate Mediator of the p53-mediated Cell Cycle Arrest at the G2 Phase

A novel gene, Reprimo, in which induction in cells exposed to X-irradiation is dependent on p53 expression, has been isolated. Ectopic p53 expression results in the induction of its mRNA. Reprimo is a highly glycosylated protein and, when ectopically expressed, it is localized in the cytoplasm and i...

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Bibliographic Details
Published in:The Journal of biological chemistry 2000-07, Vol.275 (30), p.22627-22630
Main Authors: Ohki, Rieko, Nemoto, Jiro, Murasawa, Hideki, Oda, Eri, Inazawa, Johji, Tanaka, Nobuyuki, Taniguchi, Tadatsugu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
CDC2 Protein Kinase - metabolism
Cell Cycle - physiology
Cell Cycle Proteins - chemistry
Cell Cycle Proteins - genetics
Cell Line
Cyclin B - metabolism
Cyclin B1
DNA, Complementary
G2 Phase
Genes, Suppressor
Glycoproteins - chemistry
Glycoproteins - genetics
Humans
Molecular Sequence Data
Sequence Homology, Amino Acid
Tumor Suppressor Protein p53 - physiology
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Summary:A novel gene, Reprimo, in which induction in cells exposed to X-irradiation is dependent on p53 expression, has been isolated. Ectopic p53 expression results in the induction of its mRNA. Reprimo is a highly glycosylated protein and, when ectopically expressed, it is localized in the cytoplasm and induces G2 arrest of the cell cycle. In the arrested cells, both Cdc2 activity and nuclear translocation of cyclin B1 are inhibited, suggesting the involvement of Reprimo in the Cdc2·cyclin B1 regulation pathway. Thus, Reprimo may be a new member involved in the regulation of p53-dependent G2 arrest of the cell cycle.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.C000235200