Identification of Human Autoantibodies to the Transcriptional Repressor ZF5

ZF5 was originally cloned as a transcriptional repressor on the mouse c-myc promoter. It contains the Kruppel-type zinc fingers and a conserved POZ domain, which is found in a growing number of zinc finger proteins and mediate protein-protein interactions. Autoantibodies against transcription factor...

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Bibliographic Details
Published in:Journal of autoimmunity 2000-08, Vol.15 (1), p.75-80
Main Authors: Yanagidani, Akifumi, Matsuoka, Masao, Yokoro, Ken, Tanaka, Hideo, Numoto, Michitaka
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antibody Specificity
Antigen-Antibody Reactions
Autoantibodies - immunology
Autoantigens - immunology
Biological and medical sciences
DNA-Binding Proteins - blood
DNA-Binding Proteins - immunology
Epitopes - blood
Epitopes - immunology
Epitopes - isolation & purification
General aspects
Humans
Immune Sera - metabolism
Immunopathology
Kruppel-Like Transcription Factors
Medical sciences
Molecular Sequence Data
Repressor Proteins - blood
Repressor Proteins - immunology
Transcription Factors - immunology
ZF5 antigen
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Summary:ZF5 was originally cloned as a transcriptional repressor on the mouse c-myc promoter. It contains the Kruppel-type zinc fingers and a conserved POZ domain, which is found in a growing number of zinc finger proteins and mediate protein-protein interactions. Autoantibodies against transcription factors are sometimes found in sera from patients with high levels of anti-nuclear antibody (ANA). Using Western blotting with ZF5 and sera of autoimmune disease, we detected one serum, named M6 serum, which contains the antibody against a transcriptional repressor ZF5. The confirmed epitope was specific to ZF5 and was not reactive to the other analogous factors: BCL-6, ZID, and Sp1. This epitope also has a molecular mimicry of the viral proteins. From these results, we predict that viral protein which mimics host ZF5 antigen triggers self-reactive T cell clones and induces the autoantibody in M6 serum after the destruction of host tissues.
ISSN:0896-8411
1095-9157
DOI:10.1006/jaut.2000.0385