Effect of conjugation methodology on the immunogenicity and protective efficacy of meningococcal group C polysaccharide–P64k protein conjugates

Neisseria meningitidis serogroup C polysaccharide (CCPS) was conjugated to the carrier protein P64k using two different conjugation procedures, condensation mediated by carbodiimide with adipic acid dihydrazide as spacer and the reductive amination method. BALB/c mice were immunized with the resulta...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 2004-04, Vol.40 (3), p.193-199
Main Authors: Carmenate, Tania, Canaán, Leonardo, Álvarez, Anabel, Delgado, Maité, González, Sonia, Menéndez, Tamara, Rodés, Lorenzo, Guillén, Gerardo
Format: Article
Language:English
Subjects:
Adipates
Amination
Animals
Antibodies, Bacterial - blood
Bacterial Outer Membrane Proteins - chemistry
Bacterial Outer Membrane Proteins - immunology
Bacteriology
Biological and medical sciences
Carbodiimides
Colony Count, Microbial
Disease Models, Animal
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunoglobulin G - blood
Meningococcal Infections - immunology
Meningococcal Infections - prevention & control
Meningococcal vaccine
Meningococcal Vaccines - chemistry
Meningococcal Vaccines - immunology
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Molecular Weight
Neisseria meningitidis
Neisseria meningitidis, Serogroup C - growth & development
Neisseria meningitidis, Serogroup C - immunology
Polysaccharide conjugate
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - immunology
Rats
Tissues, organs and organisms biophysics
Vaccines, Conjugate - chemistry
Vaccines, Conjugate - immunology
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Summary:Neisseria meningitidis serogroup C polysaccharide (CCPS) was conjugated to the carrier protein P64k using two different conjugation procedures, condensation mediated by carbodiimide with adipic acid dihydrazide as spacer and the reductive amination method. BALB/c mice were immunized with the resultant polysaccharide–protein conjugates and the immune response was evaluated. All conjugates assayed generated at least 10-fold higher antibody titers than the free polysaccharide. The reductive amination method rendered the best conjugate (CCPS–P64k R) that was able to elicit antibody titers statistically higher than the titer elicited by the plain CCPS ( P
ISSN:0928-8244
1574-695X
DOI:10.1016/S0928-8244(03)00346-8