Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response

Respiratory syncytial virus (RSV) is one of the most important pathogens of infancy and early childhood. Here a fruit-based edible subunit vaccine against RSV was developed by expressing the RSV fusion (F) protein gene in transgenic tomato plants. The F-gene was expressed in ripening tomato fruit un...

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Bibliographic Details
Published in:Transgenic research 2000-04, Vol.9 (2), p.127-135
Main Authors: SANDHU, J. S, KRASNYANSKI, S. F, DOMIER, L. L, KORBAN, S. S, OSADJAN, M. D, BUETOW, D. E
Format: Article
Language:English
Subjects:
Administration, Oral
AF protein
Animals
Biological and medical sciences
Biotechnology
F protein
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Immunization
Immunoglobulin A - blood
Immunoglobulin G - blood
Industrial applications and implications. Economical aspects
Lycopersicon esculentum
Lycopersicon esculentum - genetics
Lycopersicon esculentum - metabolism
Medical research
Mice
Mice, Inbred BALB C
Plants, Genetically Modified
Plasmids - genetics
Production of active biomolecules
Respiratory syncytial virus
Respiratory Syncytial Virus Infections - prevention & control
Respiratory Syncytial Virus Vaccines - administration & dosage
Respiratory Syncytial Virus Vaccines - immunology
Respiratory Syncytial Viruses - immunology
T-Lymphocytes, Helper-Inducer - immunology
Tomatoes
Vaccines, Edible - immunology
Vaccines, Subunit - immunology
Vaccins
Viral Proteins - biosynthesis
Viral Proteins - genetics
Viral Proteins - immunology
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Summary:Respiratory syncytial virus (RSV) is one of the most important pathogens of infancy and early childhood. Here a fruit-based edible subunit vaccine against RSV was developed by expressing the RSV fusion (F) protein gene in transgenic tomato plants. The F-gene was expressed in ripening tomato fruit under the control of the fruit-specific E8 promoter. Oral immunization of mice with ripe transgenic tomato fruits led to the induction of both serum and mucosal RSV-F specific antibodies. The ratio of immunoglobulin subclasses produced in response to immunization suggested that a type 1 T-helper cell immune response was preferentially induced. Serum antibodies showed an increased titer when the immunized mice were exposed to inactivated RSV antigen.
ISSN:0962-8819
1573-9368
DOI:10.1023/A:1008979525909