Characterization of 3-D collagen hydrogels for functional cell-based biosensing

To address the growing demand for functional cell-based assay technologies with accelerated drug discovery applications, we have proposed the use of human neuroblastoma cells (IMR-32) immobilized in three-dimensional (3-D) collagen hydrogel matrices. The gel protects weakly adherent cells from fluid...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2004-04, Vol.19 (9), p.1075-1088
Main Authors: CHEN MAO, KISAALITA, William S
Format: Article
Language:English
Subjects:
Biocompatible Materials
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Collagen
Extracellular Matrix
Fundamental and applied biological sciences. Psychology
Humans
Hydrogels
Materials Testing
Membrane Potentials
Methods. Procedures. Technologies
Microscopy, Confocal
Microscopy, Phase-Contrast
Patch-Clamp Techniques
Various methods and equipments
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Summary:To address the growing demand for functional cell-based assay technologies with accelerated drug discovery applications, we have proposed the use of human neuroblastoma cells (IMR-32) immobilized in three-dimensional (3-D) collagen hydrogel matrices. The gel protects weakly adherent cells from fluid mechanical forces while providing a more physiologically relevant 3-D environment. Hydrogels made up of collagen, between 0.5 and 1.0mg/ml, exhibited mechanical stability adequate to withstand fluid mechanical forces (
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2003.10.008