Statherin is an in vivo pellicle constituent: identification and immuno-quantification

Recently, we demonstrated that anti-statherin monoclonal antibodies could be generated upon immunisation of mice with in vivo formed human acquired enamel pellicle, indicating that statherin is a constituent of pellicle. To gain insight in the in vivo adsorption behaviour of statherin we tested the...

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Bibliographic Details
Published in:Archives of oral biology 2004-05, Vol.49 (5), p.379-385
Main Authors: Li, J, Helmerhorst, E.J, Yao, Y, Nunn, M.E, Troxler, R.F, Oppenheim, F.G
Format: Article
Language:English
Subjects:
Acquired enamel pellicle
Adsorption
Adult
Animals
Antibodies, Monoclonal
Capture ELISA
Dental Enamel Proteins - analysis
Dentistry
Electrophoresis, Agar Gel - methods
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Male
Mice
Middle Aged
Quantification
Saliva
Saliva - chemistry
Salivary Proteins and Peptides - analysis
Salivary Proteins and Peptides - immunology
Statherin
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Summary:Recently, we demonstrated that anti-statherin monoclonal antibodies could be generated upon immunisation of mice with in vivo formed human acquired enamel pellicle, indicating that statherin is a constituent of pellicle. To gain insight in the in vivo adsorption behaviour of statherin we tested the abundance of statherin in pellicle and investigated the relationship between statherin and protein levels in salivary secretions and pellicle using a capture ELISA. Statherin levels were approximately 20-fold higher in parotid and submandibular–sublingual secretions than in cleared whole saliva supernatant or pellicle, suggesting the rapid degradation of statherin in the oral cavity. A strong positive correlation was observed between statherin and protein levels in pellicle but not in saliva indicating that statherin and protein adsorption to pellicle are related processes. This indicates that statherin represents the integral part of proteins that constitute the pellicle structure and may play a key role in its formation.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2004.01.002