‘LE Cells’ Result from Phagocytosis of Apoptotic Bodies Induced by Antinuclear Antibodies

Lupus erythematosus (LE) cells are believed to represent phagocytosis by granulocytes of cell nuclei whose DNA has been ‘depolymerized’ and opsonized by serum factors, most likely antinuclear antibodies and C3b. Since it is known that certain antinuclear antibodies are capable of inducing apoptosis...

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Bibliographic Details
Published in:Journal of autoimmunity 2000-08, Vol.15 (1), p.15-20
Main Authors: Schmidt-Acevedo, Sylvia, Pérez-Romano, Beatriz, Ruiz-Argüelles, Alejandro
Format: Article
Language:English
Subjects:
Animals
Antibodies, Antinuclear - pharmacology
antinuclear antibodies
antinuclear antibodies, apoptosis, LE cells, nuclear antibodies, phagocytosis
Apoptosis - immunology
Biological and medical sciences
Cells, Cultured
Fluorescent Antibody Technique
General aspects
Humans
Immunopathology
In Situ Nick-End Labeling
Lupus Erythematosus, Systemic - blood
Lupus Erythematosus, Systemic - immunology
Lupus Erythematosus, Systemic - pathology
Medical sciences
Mice
Mice, Inbred NZB
Micronuclei, Chromosome-Defective - chemistry
Micronuclei, Chromosome-Defective - immunology
Neutrophils - chemistry
Neutrophils - immunology
Neutrophils - pathology
Phagocytosis - immunology
Rosette Formation
Tumor Cells, Cultured
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Description
Summary:Lupus erythematosus (LE) cells are believed to represent phagocytosis by granulocytes of cell nuclei whose DNA has been ‘depolymerized’ and opsonized by serum factors, most likely antinuclear antibodies and C3b. Since it is known that certain antinuclear antibodies are capable of inducing apoptosis after intracellular penetration; and that the resulting apoptotic bodies can be ingested by non-professional phagocytes, we decided to investigate the possibility that LE cells could result from the phagocytosis of apoptotic bodies induced by antinuclear antibodies. We demonstrate herein, through different methodological approaches, that the ingested material within LE cells corresponds to apoptotic bodies, and that the LE cell phenomenon can be reproduced, in the absence of other serum factors, by penetrating murine monoclonal anti DNA antibodies.
ISSN:0896-8411
1095-9157
DOI:10.1006/jaut.2000.0381