Testing of “special base” columns in reversed-phase liquid chromatography: A rational approach considering solvent effects

A methodology for building a chromatographic test aiming at characterizing special base stationary phases was described. Instead of choosing its conditions a priori, a “full” comprehensive test based on extended running conditions was performed on a 12 column set. The conditions were carefully chose...

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Bibliographic Details
Published in:Journal of Chromatography A 2004-03, Vol.1030 (1-2), p.135-147
Main Authors: Le Mapihan, K, Vial, J, Jardy, A
Format: Article
Language:English
Subjects:
Analysis
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid - methods
Cluster Analysis
Column testing
Drugs
Exact sciences and technology
General pharmacology
Hydrogen-Ion Concentration
Medical sciences
Other chromatographic methods
Pharmacology. Drug treatments
Principal component analysis
Solvent effects
Solvents
Stationary phases, LC
Online Access:Get full text
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Summary:A methodology for building a chromatographic test aiming at characterizing special base stationary phases was described. Instead of choosing its conditions a priori, a “full” comprehensive test based on extended running conditions was performed on a 12 column set. The conditions were carefully chosen from their ability to take into account the solvent and the pH effects. Principal component analysis (PCA) has been combined to hierarchical cluster analysis both to provide interpreted classifications and to reduce drastically the test itself by eliminating redundant information. The final reduced test can be considered optimal because the minimized set of test conditions allows to provide as much information as in the initial full test.
ISSN:0021-9673
DOI:10.1016/j.chroma.2003.10.099