Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic mice

The ability to restrict gene expression or disruption to specific regions of the brain would enhance understanding of the molecular basis for brain development and function. For this purpose, brain region‐restricted promoters are essential. Here we report the isolation of a DNA fragment containing t...

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Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2004-03, Vol.38 (3), p.130-138
Main Authors: Iwasato, Takuji, Nomura, Ryochi, Ando, Reiko, Ikeda, Toshio, Tanaka, Mika, Itohara, Shigeyoshi
Format: Article
Language:English
Subjects:
Animals
central nervous system
Chromosomes, Artificial, P1 Bacteriophage - physiology
Cre/loxP recombination
Emx1 promoter
Female
Gene Expression Regulation, Developmental - genetics
Homeodomain Proteins - genetics
Integrases - genetics
Integrases - metabolism
Lac Operon
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic - embryology
Mice, Transgenic - genetics
Mice, Transgenic - growth & development
P1-derived artificial chromosome
Promoter Regions, Genetic
Recombination, Genetic
Telencephalon - physiology
Transcription Factors
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Summary:The ability to restrict gene expression or disruption to specific regions of the brain would enhance understanding of the molecular basis for brain development and function. For this purpose, brain region‐restricted promoters are essential. Here we report the isolation of a DNA fragment containing the Emx1 gene promoter, which is responsible for dorsal telencephalon‐specific expression. The Cre recombinase gene was inserted into a mouse PAC (P1‐derived artificial chromosome) Emx1‐locus clone (PAC‐Emx1#1 clone) and utilized to generate three transgenic mouse lines. In all three lines, especially Tg3, Cre‐mediated recombination was highly restricted to Emx1‐expressing cell lineages, from embryonic stages to adulthood. Immunohistochemical analyses showed that Cre protein is expressed in the dorsal telencephalon in all three lines in adulthood. Thus, the PAC‐Emx1#1 clone contains essentially all regulatory elements necessary for Emx1 gene expression. Our results suggest that Emx1‐Cre Tg3 mice and the PAC‐Emx1#1 clone constitute powerful tools for dorsal telencephalon‐specific gene manipulation. genesis 38:130–138, 2004. © 2004 Wiley‐Liss, Inc.
ISSN:1526-954X
1526-968X
DOI:10.1002/gene.20009