Comparative study of electron mediators used in the electrochemical oxidation of NADH

A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophen...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2004-05, Vol.19 (10), p.1131-1138
Main Authors: PRIETO-SIMON, Beatriu, FABREGAS, Esteve
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
NAD - metabolism
Oxidation-Reduction
Various methods and equipments
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Summary:A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2003.10.010