Solid-phase microextraction coupled with high-performance liquid chromatography for the analysis of heterocyclic aromatic amines

Solid-phase microextraction (SPME) coupled with high-performance liquid chromatography (HPLC) with UV diode array detection (DAD) for the analysis of heterocyclic aromatic amines (HAs) is described. Four kinds of fiber coatings: Carbowax–templated resin (CW–TPR), Carbowax–divinylbenzene (CW–DVB), po...

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Bibliographic Details
Published in:Journal of Chromatography A 2004-03, Vol.1030 (1-2), p.87-93
Main Authors: Cárdenes, Lourdes, Ayala, Juan H, Afonso, Ana M, González, Venerando
Format: Article
Language:English
Subjects:
Amines
Amines - analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Food analysis
Food industries
Fundamental and applied biological sciences. Psychology
heterocyclic aromatic
Heterocyclic Compounds - analysis
Meat and meat product industries
Solid-phase microextraction
Spectrophotometry, Ultraviolet
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Summary:Solid-phase microextraction (SPME) coupled with high-performance liquid chromatography (HPLC) with UV diode array detection (DAD) for the analysis of heterocyclic aromatic amines (HAs) is described. Four kinds of fiber coatings: Carbowax–templated resin (CW–TPR), Carbowax–divinylbenzene (CW–DVB), poly(dimethylsiloxane)–divinylbenzene (PDMS–DVB) and polyacrylate (PA) were evaluated for extraction of nine most biologically active heterocyclic aromatic amines. Different parameters affecting to the microextraction and determination of HAs were studied, such as absorption and desorption time, desorption mode, composition of the solvent for desorption, pH, ionic strength, and percentage of methanol in the sample. To determine these amines in food samples a new simplified procedure is proposed, consisting of treatment of the sample with methanolic NaOH prior microextraction by CW–TPR fiber coating and HPLC–DAD determination. The advantages of this new method are the reduced amounts of time and organic solvents required.
ISSN:0021-9673
DOI:10.1016/j.chroma.2003.10.040