Host Gene Regulation During Coxsackievirus B3 Infection in Mice: Assessment by Microarrays

Host genetic responses that characterize enteroviral myocarditis have not yet been determined. The injurious and inflammatory process in heart muscle may reflect host responses of benefit to the virus and ultimately result in congestive heart failure and dilated cardiomyopathy. On the other hand, ho...

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Bibliographic Details
Published in:Circulation research 2000-08, Vol.87 (4), p.328-334
Main Authors: Taylor, Lydia A, Carthy, Christopher M, Yang, Decheng, Saad, Kareem, Wong, Donald, Schreiner, George, Stanton, Lawrence W, McManus, Bruce M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Coxsackievirus Infections - genetics
Coxsackievirus Infections - immunology
Coxsackievirus Infections - metabolism
Energy Metabolism - physiology
Enterovirus - genetics
Experimental viral diseases and models
Gene Expression Regulation, Viral - immunology
Heart - virology
HeLa Cells
Humans
Immunocompetence
In Situ Hybridization
Infectious diseases
Medical sciences
Mice
Mice, Inbred A
Myocarditis - immunology
Myocarditis - metabolism
Myocarditis - virology
Oligonucleotide Array Sequence Analysis
RNA, Messenger - analysis
RNA, Viral - analysis
Time Factors
Viral diseases
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Summary:Host genetic responses that characterize enteroviral myocarditis have not yet been determined. The injurious and inflammatory process in heart muscle may reflect host responses of benefit to the virus and ultimately result in congestive heart failure and dilated cardiomyopathy. On the other hand, host responses within the myocardium may secure the host against acute or protracted damage. To investigate the nature of modified gene expression in comparison with normal tissue, mRNA species were assessed in myocardium using cDNA microarray technology at days 3, 9, and 30 after infection. Of 7000 clones initially screened, 169 known genes had a level of expression significantly different at 1 or more postinfection time points as compared with baseline. The known regulated genes were sorted according to their functional groups and normalized expression patterns and, subsequently, interpreted in the context of viremic, inflammatory, and healing phases of the myocarditic process.
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.87.4.328