Effects of ions and detergents in drug partition chromatography on liposomes

We have determined drug partitioning into phospholipid bilayers by immobilized-liposome chromatography (ILC). Electrostatic effects on the drug partitioning were observed on neutral bilayers at low ionic strength. The size of the counterions affected the partitioning. When liposomes were supplemente...

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Bibliographic Details
Published in:Journal of Chromatography A 2004-03, Vol.1030 (1-2), p.273-278
Main Authors: Boija, Elisabet, Lundquist, Anna, Martı́nez Pla, Juan José, Engvall, Caroline, Lundahl, Per
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid - methods
Detergents
Drug partitioning
Drugs
Electrostatic effects
Exact sciences and technology
Fatty Acids - chemistry
General pharmacology
Immobilized-liposome chromatography
Ionic strength
Lipid Bilayers
Liposomes
Medical sciences
Osmolar Concentration
Other chromatographic methods
Pharmaceutical Preparations - isolation & purification
Pharmacology. Drug treatments
Phospholipid bilayers
Phospholipids - chemistry
Physicochemical properties. Structure-activity relationships
Surfactants
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Summary:We have determined drug partitioning into phospholipid bilayers by immobilized-liposome chromatography (ILC). Electrostatic effects on the drug partitioning were observed on neutral bilayers at low ionic strength. The size of the counterions affected the partitioning. When liposomes were supplemented with ionic detergents the partitioning of charged drugs was strongly affected, allowing complete separation of drugs of different charges which showed similar retention on neutral bilayers. Partial separation was obtained on bilayers containing fatty acid. Detergent ions or fatty acid inserted into phospholipid bilayers affected the partitioning of drugs much more than did free ions or phospholipid head group charges.
ISSN:0021-9673
DOI:10.1016/j.chroma.2003.11.060