Regulation of Binding of Lamin B Receptor to Chromatin by SR Protein Kinase and cdc2 Kinase in Xenopus Egg Extracts

• Published in: The Journal of biological chemistry 2004-03, Vol.279 (13), p.13265-13271
• Main Authors: Takano, Makoto, Koyama, Yuhei, Ito, Hiromi, Hoshino, Satomi, Onogi, Hiroshi, Hagiwara, Masatoshi, Furukawa, Kazuhiro, Horigome, Tsuneyoshi
• Format: Article
• Language: English
• Subjects: Animals; CDC2 Protein Kinase - metabolism; Cell Nucleus - metabolism; Cellulose - chemistry; Chromatin - chemistry; Chromatin - metabolism; DNA - chemistry; Gene Expression Regulation, Enzymologic; Glutathione Transferase - metabolism; Humans; Lamin B Receptor; Mitosis; Mutation; Oocytes - enzymology; Oocytes - metabolism; Peptide Mapping; Phosphorylation; Protein Binding; Protein Serine-Threonine Kinases - metabolism; Purines - pharmacology; Receptors, Cytoplasmic and Nuclear - chemistry; Receptors, Cytoplasmic and Nuclear - metabolism; Recombinant Proteins - chemistry; Roscovitine; S Phase; Serine - chemistry; Trypsin - pharmacology; Xenopus
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M308854200

Participation of multiple kinases in regulation of the binding of lamin B receptor (LBR) to chromatin was suggested previously (Takano, M., Takeuchi, M., Ito, H., Furukawa, K., Sugimoto, K., Omata, S., and Horigome, T. (2002) Eur. J. Biochem. 269, 943-953). To identify these kinases, regulation of the binding of the nucleoplasmic region (NK, amino acid residues 1-211) of LBR to sperm chromatin was studied using a cell cycle-dependent Xenopus egg extract in vitro. The binding was stimulated on specific phosphorylation of the NK fragment by an S-phase egg extract. Protein depletion with beads bearing SF2/ASF, which binds SR protein kinases, abolished this stimulation, suggesting that an SR protein kinase(s) is responsible for the activation of LBR. This was confirmed by direct phosphorylation and activation with recombinant SR protein-specific kinase 1. The binding of the NK fragment to chromatin pretreated with an S-phase extract was suppressed by incubation with an M-phase extract. Enzyme inhibitor experiments revealed that multiple kinases participate in the suppression. One of these kinases was shown to be cdc2 kinase using a specific inhibitor, roscovitine, and protein depletion with beads bearing p13, which specifically binds cdc2 kinase. Experiments involving a mutant NK fragment showed that the phosphorylation of serine 71 by cdc2 kinase is responsible for the suppression.