Effect of Aminoguanidine in Ligature-induced Periodontitis in Rats

The role of nitric oxide and reactive oxygen species is well-demonstrated in inflammation. In this study, we evaluated the effect of aminoguanidine, a nitric oxide synthase inhibitor, in a rat model of periodontitis. We induced periodontitis in rats by placing a piece of 2/0 braided silk around the...

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Published in:Journal of dental research 2004-04, Vol.83 (4), p.343-348
Main Authors: Di Paola, R., Marzocco, S., Mazzon, E., Dattola, F., Rotondo, F., Britti, D., De Majo, M., Genovese, T., Cuzzocrea, S.
Format: Article
Language:English
Subjects:
Alveolar Bone Loss - etiology
Animals
Dentistry
Disease Models, Animal
Enzyme Inhibitors - pharmacology
Gingiva - enzymology
Gingiva - pathology
Guanidines - pharmacology
Ligation - adverse effects
Lipid Peroxidation - physiology
Male
Mouth Mucosa - enzymology
Mouth Mucosa - pathology
Neutrophil Infiltration - physiology
Nitric Oxide - metabolism
Nitric Oxide Synthase - antagonists & inhibitors
Periodontitis - enzymology
Periodontitis - etiology
Poly(ADP-ribose) Polymerases - metabolism
Random Allocation
Rats
Rats, Sprague-Dawley
Tyrosine - analogs & derivatives
Tyrosine - metabolism
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Summary:The role of nitric oxide and reactive oxygen species is well-demonstrated in inflammation. In this study, we evaluated the effect of aminoguanidine, a nitric oxide synthase inhibitor, in a rat model of periodontitis. We induced periodontitis in rats by placing a piece of 2/0 braided silk around the lower left 1st molar. At day 8, the gingivomucosal tissue encircling the mandibular 1st molar was removed for biochemical and histological analysis. Ligation significantly increased inducible nitric oxide synthase activity and expression, and damaged tissue revealed increased neutrophil infiltration, lipid peroxidation, and positive staining for nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Aminoguanidine (100 mg/kg i.p., daily for 8 days) treatment significantly reduced all these inflammatory parameters, indicating that it protects against the tissue damage associated with periodontitis by reducing nitric oxide production and oxidative stress.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910408300414