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Suppression of natural killer cell activity by splenocyte transplantation in a rat model of endometriosis
BACKGROUND: One immune characteristic of endometriosis is a decrease in natural killer (NK) cell activity. This study was performed to determine whether an abnormal immune reaction in an endometriosis animal model could be transferred to an animal of the same species. METHODS: An endometriosis model...
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| Published in: | Human reproduction (Oxford) 2002-06, Vol.17 (6), p.1453-1458 |
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| container_issue | 6 |
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| container_title | Human reproduction (Oxford) |
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| creator | Ota, Hirotaka Rong, Huang Igarashi, Shinichi Tanaka, Toshinobu |
| description | BACKGROUND: One immune characteristic of endometriosis is a decrease in natural killer (NK) cell activity. This study was performed to determine whether an abnormal immune reaction in an endometriosis animal model could be transferred to an animal of the same species. METHODS: An endometriosis model was prepared using 8 week old female rats by grafting a small section of one uterine horn onto the mesentery, followed 4 weeks later by removal of the spleen and remaining uterine horn. Splenocytes, that had been depleted of macrophages were injected via the tail vein, and NK cell activity of splenocytes was determined 4 days later. The uterus was simultaneously investigated immunohistochemically for immune cells. There was a control group (untreated; group 1), a control–splenocyte injection group (group 2), an experimental endometriosis model group (group 3) and an endometriosis model splenocyte injection group (group 4). RESULTS: Splenocyte NK cell activity was decreased in group 3 to 42.0% of that of group 1 and in group 4 to 38.9%. Immunohistologically, the number of NK cells in groups 3 and 4 markedly decreased to 62.0 and 55.1% of group 1 respectively. CONCLUSION: It was demonstrated that abnormal immunity caused by allograft of immune cells could recur in an endometriosis rat model. |
| doi_str_mv | 10.1093/humrep/17.6.1453 |
| format | article |
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This study was performed to determine whether an abnormal immune reaction in an endometriosis animal model could be transferred to an animal of the same species. METHODS: An endometriosis model was prepared using 8 week old female rats by grafting a small section of one uterine horn onto the mesentery, followed 4 weeks later by removal of the spleen and remaining uterine horn. Splenocytes, that had been depleted of macrophages were injected via the tail vein, and NK cell activity of splenocytes was determined 4 days later. The uterus was simultaneously investigated immunohistochemically for immune cells. There was a control group (untreated; group 1), a control–splenocyte injection group (group 2), an experimental endometriosis model group (group 3) and an endometriosis model splenocyte injection group (group 4). RESULTS: Splenocyte NK cell activity was decreased in group 3 to 42.0% of that of group 1 and in group 4 to 38.9%. Immunohistologically, the number of NK cells in groups 3 and 4 markedly decreased to 62.0 and 55.1% of group 1 respectively. CONCLUSION: It was demonstrated that abnormal immunity caused by allograft of immune cells could recur in an endometriosis rat model.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/17.6.1453</identifier><identifier>PMID: 12042260</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adoptive Transfer ; Animals ; Biological and medical sciences ; Cytotoxicity, Immunologic ; Disease Models, Animal ; Endometriosis ; Endometriosis - immunology ; Endometriosis - pathology ; endometriosis model ; Endometrium - immunology ; Endometrium - pathology ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Humans ; Immune Tolerance ; In Vitro Techniques ; Killer Cells, Natural - immunology ; Macrophages - immunology ; Medical sciences ; natural killer cell activity ; Non tumoral diseases ; Rats ; Rats, Wistar ; Spleen - cytology ; Spleen - immunology ; Spleen - transplantation ; splenocyte ; transplantation ; Transplantation, Homologous ; Tumor Cells, Cultured</subject><ispartof>Human reproduction (Oxford), 2002-06, Vol.17 (6), p.1453-1458</ispartof><rights>European Society of Human Reproduction and Embryology 2002</rights><rights>2002 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Jun 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-f49a2949436316dcb789665e3b00be13b4b1ed6dfd785c13da26ff9878a6d3d53</citedby><cites>FETCH-LOGICAL-c426t-f49a2949436316dcb789665e3b00be13b4b1ed6dfd785c13da26ff9878a6d3d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13712869$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12042260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ota, Hirotaka</creatorcontrib><creatorcontrib>Rong, Huang</creatorcontrib><creatorcontrib>Igarashi, Shinichi</creatorcontrib><creatorcontrib>Tanaka, Toshinobu</creatorcontrib><title>Suppression of natural killer cell activity by splenocyte transplantation in a rat model of endometriosis</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>BACKGROUND: One immune characteristic of endometriosis is a decrease in natural killer (NK) cell activity. This study was performed to determine whether an abnormal immune reaction in an endometriosis animal model could be transferred to an animal of the same species. METHODS: An endometriosis model was prepared using 8 week old female rats by grafting a small section of one uterine horn onto the mesentery, followed 4 weeks later by removal of the spleen and remaining uterine horn. Splenocytes, that had been depleted of macrophages were injected via the tail vein, and NK cell activity of splenocytes was determined 4 days later. The uterus was simultaneously investigated immunohistochemically for immune cells. There was a control group (untreated; group 1), a control–splenocyte injection group (group 2), an experimental endometriosis model group (group 3) and an endometriosis model splenocyte injection group (group 4). RESULTS: Splenocyte NK cell activity was decreased in group 3 to 42.0% of that of group 1 and in group 4 to 38.9%. Immunohistologically, the number of NK cells in groups 3 and 4 markedly decreased to 62.0 and 55.1% of group 1 respectively. CONCLUSION: It was demonstrated that abnormal immunity caused by allograft of immune cells could recur in an endometriosis rat model.</description><subject>Adoptive Transfer</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cytotoxicity, Immunologic</subject><subject>Disease Models, Animal</subject><subject>Endometriosis</subject><subject>Endometriosis - immunology</subject><subject>Endometriosis - pathology</subject><subject>endometriosis model</subject><subject>Endometrium - immunology</subject><subject>Endometrium - pathology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immune Tolerance</subject><subject>In Vitro Techniques</subject><subject>Killer Cells, Natural - immunology</subject><subject>Macrophages - immunology</subject><subject>Medical sciences</subject><subject>natural killer cell activity</subject><subject>Non tumoral diseases</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Spleen - transplantation</subject><subject>splenocyte</subject><subject>transplantation</subject><subject>Transplantation, Homologous</subject><subject>Tumor Cells, Cultured</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqNkc2L1TAUxYMozvPp3pUEQTfSN7lJe9Mux-fHKCMiKoibkDYpZqZtapKK77835T0ccOUqCfzOyb3nEPIY2A5YI85_LGOw8znIHe6grMQdsoESWcFFxe6SDeNYFwAIZ-RBjNeM5WuN98kZcFZyjmxD3OdlnoON0fmJ-p5OOi1BD_TGDYMNtLPDQHWX3C-XDrQ90DgPdvLdIVmagp7yU09Jp1XtJqpp0ImO3thhNbOT8aNNwfno4kNyr9dDtI9O55Z8ffP6y_6yuPr49t3-4qroSo6p6MtG86ZsSoEC0HStrBvEyoqWsdaCaMsWrEHTG1lXHQijOfZ9U8taoxGmElvy_Og7B_9zsTGp0cV1Dz1Zv0QlQUohG8zg03_Aa7-EKc-mOOScoMlDbAk7Ql3wMQbbqzm4UYeDAqbWDtSxAwVSoVo7yJInJ9-lHa25FZxCz8CzE6Bjp4c-59i5eMsJCbzGJnMvjpxf5v_5tjjSLib7-y-vw43CvHClLr99Vwz37z_Ur16qT-IPKT2vSQ</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Ota, Hirotaka</creator><creator>Rong, Huang</creator><creator>Igarashi, Shinichi</creator><creator>Tanaka, Toshinobu</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>Suppression of natural killer cell activity by splenocyte transplantation in a rat model of endometriosis</title><author>Ota, Hirotaka ; Rong, Huang ; Igarashi, Shinichi ; Tanaka, Toshinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-f49a2949436316dcb789665e3b00be13b4b1ed6dfd785c13da26ff9878a6d3d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adoptive Transfer</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cytotoxicity, Immunologic</topic><topic>Disease Models, Animal</topic><topic>Endometriosis</topic><topic>Endometriosis - immunology</topic><topic>Endometriosis - pathology</topic><topic>endometriosis model</topic><topic>Endometrium - immunology</topic><topic>Endometrium - pathology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immune Tolerance</topic><topic>In Vitro Techniques</topic><topic>Killer Cells, Natural - immunology</topic><topic>Macrophages - immunology</topic><topic>Medical sciences</topic><topic>natural killer cell activity</topic><topic>Non tumoral diseases</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Spleen - transplantation</topic><topic>splenocyte</topic><topic>transplantation</topic><topic>Transplantation, Homologous</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ota, Hirotaka</creatorcontrib><creatorcontrib>Rong, Huang</creatorcontrib><creatorcontrib>Igarashi, Shinichi</creatorcontrib><creatorcontrib>Tanaka, Toshinobu</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ota, Hirotaka</au><au>Rong, Huang</au><au>Igarashi, Shinichi</au><au>Tanaka, Toshinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of natural killer cell activity by splenocyte transplantation in a rat model of endometriosis</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>17</volume><issue>6</issue><spage>1453</spage><epage>1458</epage><pages>1453-1458</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND: One immune characteristic of endometriosis is a decrease in natural killer (NK) cell activity. This study was performed to determine whether an abnormal immune reaction in an endometriosis animal model could be transferred to an animal of the same species. METHODS: An endometriosis model was prepared using 8 week old female rats by grafting a small section of one uterine horn onto the mesentery, followed 4 weeks later by removal of the spleen and remaining uterine horn. Splenocytes, that had been depleted of macrophages were injected via the tail vein, and NK cell activity of splenocytes was determined 4 days later. The uterus was simultaneously investigated immunohistochemically for immune cells. There was a control group (untreated; group 1), a control–splenocyte injection group (group 2), an experimental endometriosis model group (group 3) and an endometriosis model splenocyte injection group (group 4). RESULTS: Splenocyte NK cell activity was decreased in group 3 to 42.0% of that of group 1 and in group 4 to 38.9%. Immunohistologically, the number of NK cells in groups 3 and 4 markedly decreased to 62.0 and 55.1% of group 1 respectively. CONCLUSION: It was demonstrated that abnormal immunity caused by allograft of immune cells could recur in an endometriosis rat model.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>12042260</pmid><doi>10.1093/humrep/17.6.1453</doi><tpages>6</tpages></addata></record> |
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| subjects | Adoptive Transfer Animals Biological and medical sciences Cytotoxicity, Immunologic Disease Models, Animal Endometriosis Endometriosis - immunology Endometriosis - pathology endometriosis model Endometrium - immunology Endometrium - pathology Female Female genital diseases Gynecology. Andrology. Obstetrics Humans Immune Tolerance In Vitro Techniques Killer Cells, Natural - immunology Macrophages - immunology Medical sciences natural killer cell activity Non tumoral diseases Rats Rats, Wistar Spleen - cytology Spleen - immunology Spleen - transplantation splenocyte transplantation Transplantation, Homologous Tumor Cells, Cultured |
| title | Suppression of natural killer cell activity by splenocyte transplantation in a rat model of endometriosis |
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