Cell kinetic balance in gastric mucosa with intestinal metaplasia after Helicobacter pylori eradication: 2-year follow-up study

• Published in: Digestive and liver disease 2004-03, Vol.36 (3), p.178-186
• Main Authors: Wambura, C, Aoyama, N, Shirasaka, D, Kuroda, K, Watanabe, Y, Miki, I, Tamura, T, Kasuga, M
• Format: Article
• Language: English
• Subjects: Adult; Aged; Anti-Bacterial Agents - therapeutic use; Apoptosis - drug effects; Apoptosis - genetics; Cell Division - drug effects; Cell proliferation and apoptosis; Endoscopes, Gastrointestinal; Female; Follow-Up Studies; Gastric Mucosa - chemistry; Gastric Mucosa - drug effects; Gastric Mucosa - pathology; Helicobacter Infections - drug therapy; Helicobacter Infections - metabolism; Helicobacter Infections - pathology; Helicobacter pylori - drug effects; Helicobacter pylori eradication; Humans; Immunohistochemistry; Intestinal metaplasia; Ki-67 Antigen - analysis; Kinetics; Male; Metaplasia; Middle Aged
• ISSN: 1590-8658; 1878-3562
• DOI: 10.1016/j.dld.2003.10.014

Background. Proliferation and apoptosis events are altered in Helicobacter pylori infection. However, whether H. pylori eradication has an effect on the disturbed kinetics in metaplastic mucosa has not been well elucidated. Aim. To investigate the effect of eradication on the gastric cell kinetics. Subjects and methods. Initially, biopsies were obtained from 74 H. pylori-infected subjects and repeated 12 and 24 months after eradication. Biopsies were immunohistochemically stained for apoptosis by single-stranded DNA, for proliferation by Ki-67 antibodies and for intestinal metaplasia MUC2, MUC5AC, MUC6 and CD10. Results. While antral apoptosis in intestinal metaplasia was significantly lower than in non-intestinal metaplasia, proliferation was significantly higher (greater and lesser curvatures, P