Mediator–Nucleosome Interaction

Mediator, a multiprotein complex involved in the regulation of RNA polymerase II transcription, binds to nucleosomes and acetylates histones. Three lines of evidence identify the Nut1 subunit of Mediator as responsible for the histone acetyltransferase (HAT) activity. An “in-gel” HAT assay reveals a...

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Bibliographic Details
Published in:Molecular cell 2000-07, Vol.6 (1), p.197-201
Main Authors: Lorch, Yahli, Beve, Jenny, Gustafsson, Claes M., Myers, Lawrence C., Kornberg, Roger D.
Format: Article
Language:English
Subjects:
Acetyltransferases - chemistry
Acetyltransferases - genetics
Acetyltransferases - metabolism
Amino Acid Sequence
Base Sequence
DNA Primers - genetics
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - metabolism
histone acetyltransferase
Histone Acetyltransferases
Macromolecular Substances
Mediator Complex
Molecular Sequence Data
Multiprotein Complexes
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Nucleosomes - metabolism
Nut1 protein
RNA Polymerase II - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
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Summary:Mediator, a multiprotein complex involved in the regulation of RNA polymerase II transcription, binds to nucleosomes and acetylates histones. Three lines of evidence identify the Nut1 subunit of Mediator as responsible for the histone acetyltransferase (HAT) activity. An “in-gel” HAT assay reveals a single band of the appropriate size. Sequence alignment shows significant similarity of Nut1 to the GCN5-related N-acetyltransferase superfamily. Finally, recombinant Nut1 exhibits HAT activity in an in-gel assay.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(05)00007-9