Effect of respiratory muscle training on GLUT-4 in the sheep diaphragm

• Published in: Medicine and science in sports and exercise 2000-08, Vol.32 (8), p.1406-1411
• Main Authors: BHANDARI, A, YING XIA, CORTRIGHT, R, DOHM, G. L, BAZZY, A. R
• Format: Article
• Language: English
• Subjects: Air breathing; Animals; Biological and medical sciences; Blotting, Northern; Blotting, Western; Breathing Exercises; Diaphragm - metabolism; Diaphragm - physiology; Fundamental and applied biological sciences. Psychology; Glucose Transporter Type 4; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics; Monosaccharide Transport Proteins - genetics; Monosaccharide Transport Proteins - metabolism; Muscle Proteins; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics; RNA, Messenger - blood; Sheep; Space life sciences; Vertebrates: respiratory system
• ISSN: 0195-9131; 1530-0315
• DOI: 10.1097/00005768-200008000-00008

Endurance exercise training is associated with enhanced glucose uptake and hence improvement in carbohydrate metabolism. Glucose transport (GLUT) membrane proteins are regulated by a variety of physiological stimuli, including exercise. In limb muscle, both acute exercise and endurance training enhance the expression of the skeletal muscle transporter, GLUT-4. The purpose of this study is to determine whether chronic loading enhances GLUT-4 expression in the diaphragm. The effect of chronic inspiratory flow resistive (IFR) loading on diaphragm GLUT-4 was studied in a model of respiratory muscle endurance training in sheep. IFR loads (resistance 50-100 cm H2O x L(-1) x s(-1)) were maintained for 3 h x d(-1), 5-6 d x wk(-1) for 3 wk. Loading was adjusted so that PaO2 was >60 Torr and PaCO2